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| 10671549 | caspase 3 | caspase 3 | 1.0 | cytochrome c release from mitochondria and caspase 3 activation also occurred. | |  |
| 10671549 | caspase 3 | caspase 3 | 1.0 | he results obtained showed that gsno mediated apoptosis in these cells is associated with a canonical sequence of events including bcl 2 p53 and p21 modulation cytochrome c release in the cytosol and caspase 3 activation. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | ac devd amc caspase 3 inhibitor i ac devd cho hoechst 33342 diethylamine nonoate nono and 3 [ _amp_#177; e ethyl 2' [ e hydroxyimino] 5 nitro 3 exenecarbamoyl]pyridine nor 4 were purchased from calbiochem. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | caspase 3 activation cells attached and detached were washed and collected by centrifugation at 700 _amp_#215; g for 5 min at 4 degreec. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | supernatants were used for caspase 3 assay in lysis buffer containing the specific substrate ac devd amc 12 micro m at 30 degreec. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | specific activity was demonstrated by the use of the caspase 3 inhibitor i ac devd cho that completely impedes the development of fluorescence due to the cleaved product. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | it has been demonstrated that p21 cleavage could be reproduced in extracts prepared from irradiated cells or by recombinant caspase 3 suggesting that a caspase like activity is responsible for this cleavage. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | b caspase 3 activity was measured fluorometrically with ac devd amc as substrate. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | proteolytic activity associated to caspases was measured by testing the cytosolic extracts for their ability to cleave the fluorimetric substrate ac devd amc which is specific for caspase 3. | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | caspase 3 activation is important in the cascade of proteolytic events of apoptosis in that it is considered one of the points of no return in the process leading to cell destruction 40 . | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | caspase 3 activation followed the same trend as cytochrome c release with higher activity in the cytosolic extracts of g93a cells than in sh sy5y and wt cells fig 3 b . | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | nono diethylamine nonoate; nor 4 3 [ _amp_#177; e ethyl 2' [ e hydroxyimino] 5 nitro 3 exenecarbamo yl]pyridine; dcf da 2' 7' dichlorodihydrofluorescein diacetate; ac devd amc ac asp glu val asp amc caspase 3 substrate ii fluorogenic; ac devd cho ac asp glu val asp cho caspase 3 inhibitor i; ros reactive oxygen species; fals familial amyotrophic lateral sclerosis; pipes piperazine n n ' bis[2 ethanesulfon | | |
| 10671549 | caspase 3 | caspase 3 | 1.0 | substrate ii fluorogenic; ac devd cho ac asp glu val asp cho caspase 3 inhibitor i; ros reactive oxygen species; fals familial amyotrophic lateral sclerosis; pipes piperazine n n ' bis[2 ethanesulfonic acid]; chaps 3 [cholamindopropyl dimethylammonio] 1 propanesulfonate | | |
| 10742195 | caspase 3 | caspase 3 | 1.0 | vallee_amp_#x2019;s group [ 3 ] has now shown that nanomolar levels of zn 2+ modulated by mt regulate the activities of metabolically critical enzymes: caspase 3 critical for initiating apoptosis was inhibited by zn 2+ with an ic 50 _amp_#x3c; 10 nm 1:1 stoichiometry ; and the ic 50 for fructose 1 6 diphosphatase aldehyde dehydrogenase and tyrosine phosphatas | | |
| 12901835 | caspase 3 | caspase 3 | 1.0 | caspase 3 activity apoalert caspase 3 colorimetric assay kit clontech palo alto usa | | |
| 12901835 | caspase 3 | caspase 3 | 1.0 | neither caspase 3 activation fig 1b nor the number of condensed nuclei fig 1c_amp_#x2013;e was found to be significantly increased in g93a mutant cells indicating that no significant spontaneous apoptotic tendency was | | |
| 15208263 | caspase 3 | caspase 3 | 1.0 | activation of caspase 1 and caspase 3 is observed also in neuroblastoma lines expressing other fals sod1s g37r g85r and i113t cocultured with glioblastoma lines expressing the corresponding mutant enzymes. | | |
| 15896810 | caspase 3 | caspase 3 | 1.0 | hsp70 has been demonstrated to inhibit caspase 3 activation caused by ceramide and also affect jun kinase and p38 kinase activation [141] . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | caspase 1 and caspase 3 activities were determined by measuring the rates of 7 amido 4 methylcoumarin amc release from synthetic caspase substrates: ac yvad amc for caspase 1 and ac devd amc for caspase 3 calbiochem . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | specific activity was demonstrated by the use of the caspase 1 and caspase 3 inhibitors ac yvad cho and ac devd cho respectively that completely block the development of fluorescence due to the cleaved product. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | caspase 3 activity was detected using a rabbit polyclonal antibody cell signaling specifically reacting with the cleaved 17/19 kda fragments of active caspase 3. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | icularly conspicuous considering that the percentage of transfected cells was estimated around 30% see materials and methods . 48 h after transfection expression of mutant sod1s induces activation of caspase 3 caspase 9 but not caspase 1 fig 1 c . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | transient transfection of control sh cells or sh/apaf1 cells with wtsod1 or g93a sod1 mutant elicits high level comparable expression of the two proteins in both cell lines fig 2 a but activation of caspase 3 is dramatically increased only in g93a expressing sh/apaf1 cells as compared to g93a transfected sh cells fig 2 b . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | etna_amp_#x2212;/_amp_#x2212; cells do not express apaf1 and the absence of this adapter provides them with a complete resistance to caspase 3 activation elicited by a classic pro apoptotic agent such as staurosporine cozzolino et al. 2004 . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | we have observed that apaf1 deletion also provides these cells with a complete resistance to activation of caspase 3 induced by several fals sod1s and observed in control etna+/+ cells 24 h after transfection with the cdna coding for the mutant protein fig 3 a . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | it is widely believed that activation of the apoptosome followed by caspase 9 and caspase 3 activation plays a central role in cell death in the nervous system yuan and yankner 2000 . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | we have observed that indeed apaf1 is a key factor in the noxious function of fals sod1: while overexpression of apaf1 exacerbates the induction of apoptosis fig 2 its removal prevents activation of caspase 3 even in the presence of cytochrome c release fig 3 and fig 4 . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | t panel: sh sy5y cells were transfected as in panel a. after 48 h equal amounts 30 _amp_#x3bc;g of clear cell lysates were incubated with 20 _amp_#x3bc;m ac yvad amc for caspase 1 and ac devd amc for caspase 3 . | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | right panel: caspase 1 caspase 3 and caspase 9 activity were also assessed by western blot. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | b cells were treated as in panel a and analyzed for caspase 3 activation 24 h after transfection. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | a left panel: caspase 3 activity was assayed in cells 24 h after transient transfection with either wtsod1 or g93a g37r g85r and i113t sod1 mutants. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | caspase 3 activity is expressed as arbitrary fluorescence units afu n _amp_#xa0;=_amp_#xa0;3 . 100 _amp_#x3bc;m zvad fmk a pan caspase inhibitor was used. | | |
| 16046141 | caspase 3 | caspase 3 | 1.0 | right panel: caspase 3 activity was also assessed by western blot. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | furthermore malonate did not induce the expression of the inos caspase 3 8 and 9 genes which have been shown to be up regulated in several models where minocycline resulted cytoprotective. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | tetracyclines can inhibit the matrix metalloproteases activity and superoxide production and can also down regulate the levels of expression of interleukin 1 beta inducible nitric oxide synthase inos caspase 3 and 1 chen et al. 2000 . | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | moreover although no modifications in the expression of the mrna's encoding for inos caspase 3 8 and 9 after malonate additions were found we observed a significant reduction of blc 2 mrna expression which was not reverted by minocycline. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | inos caspase 3 8 and 9 mrna expression levels are not modulated by malonate | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | in order to check whether these pathways were involved in malonate induced granular cell death we first analyzed the pattern of expression of inos caspase 3 8 and 9 and the antiapoptotic gene bcl 2 in malonate treated cells. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | the results shown in fig. 6 a revealed that malonate at the concentration which causes a significant decrease in cell viability does not modify the expression of the inos caspase 3 8 and 9 genes. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | additionally although malonate treatment fails to induce the mrna expression of caspase 3 8 and 9 as well as inos it decreases the mrna of the antiapoptotic protein bcl 2 an effect that was not abrogated by minocycline. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | we found no changes in the expression of the genes coding for inos caspase 3 8 and 9. | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | fig. 6._amp_#xa0;malonate does not affect inos caspase 3 8 and 9 but significantly reduces bcl 2 mrna expression levels. mrna expression in malonate treated cerebellar granular cells was quantitatively measured by real time pcr using gadph mrna levels as c | | |
| 16242643 | caspase 3 | caspase 3 | 1.0 | a lack of effects of malonate on the inos caspase 3 8 and 9 mrna expression levels. | | |
| 16406002 | caspase 3 | caspase 3 | 1.0 | in various cell lines reactive aldehyde induced apoptosis was accompanied by c jun n terminal kinase and caspase 3 activation. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | the proapoptotic proteins fas fas associated death domain caspase 8 and caspase 3 were also elevated. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | altered expression and dysfunction of bcl 2 may contribute to the activation of mitochondrial apoptosis machinery such as caspase 9 caspase 3 and cytochrome c in spinal motor neurons of als transgenic mice and humans with als guegan et al. 2001 ; inoue et al. 2003 . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | ctions were then reacted overnight at 4degreec with the primary antibodies: mouse anti fas bd biosciences san jose ca anti nitrotyrosine 4 microg/ml; upstate biotechnology lake placid ny anti cleaved caspase 3 cell signaling technology danvers ma and anti neun. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | the following primary antibodies were used: fas fadd bd bioscience franklin lakes nj cleaved caspase 3 and cleaved caspase 8 1 microg/ml; cell signaling technology . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | we found that expression of fas and fadd were increased selectively in the ventral motor neurons of g93a transgenic mice and that this led to activation of caspase 8 and caspase 3. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | in support of this li blocked activation of the fas pathway during serum deprivation induced apoptosis and attenuated motor neuron degeneration as well as activation of fas caspase 8 and caspase 3 in the spinal cords of als mice. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | c western blot analysis showing expression of cleaved caspase 8 cleaved caspase 3 and actin in lumbar segments from control or g93a mice at ages indicated a . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | levels of cleaved caspase 8 b and caspase 3 c were measured and scaled to actin mean _amp_#177; s.e.m. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | d fluorescence photomicrographs of lumbar ventral sections from control a and g93a mice b at 12 weeks of age immunolabeled with an antibody for cleaved caspase 3. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | note activation of caspase 3 in the motor neurons arrows from g93a mice. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | c western blot analysis of fadd after immunoprecipitation ip with fas antibody cleaved caspase 8 cleaved caspase 3 and actin in neuron rich cortical cell cultures deprived of serum for 12 h alone or in the presence of 1 microm neu2000 or 5 mm li . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | f western blot analysis of fas fadd cleaved caspase 8 cleaved caspase 3 and actin in lumbar segments from control and g93a transgenic mice treated with saline neu2000 30 mg/kg/days or li 200 mg/kg/days for 4 weeks starting from 8 weeks of age a . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | increased expression of the apoptosis inducing signaling complex was followed by activation of caspase 8 and caspase 3 in the lumbar spinal cord fig 2c . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | the active form of caspase 3 was observed in spinal motor neurons from g93a mice fig 2d . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | these findings suggest that fas fadd caspase 8 and caspase 3 are activated in spinal motor neurons and mediate subsequent neuronal apoptosis in als mice. | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | interaction of fadd with fas cleaved caspase 8 and cleaved caspase 3 were all increased in neuron rich cortical cell cultures deprived of serum for 8 h and these changes were blocked by the addition of li but not neu2000 fig 3c . | | |
| 17105868 | caspase 3 | caspase 3 | 1.0 | daily administration of neu2000 in the diet slightly but statistically insignificantly attenuated the increase in fas fadd and cleaved caspase 8 and caspase 3 in the lumbar spinal cords of g93a transgenic mice at 12 weeks of age fig 3f . | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | furthermore depletion of gsh decreased mitochondrial function facilitated apoptosis inducing factor aif translocation cytochrome c release and caspase 3 activation and consequently led to motor neuron like cell apoptosis. | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | in an als like transgenic mouse model overexpressing mutant g93a sod1 gene we showed that the reduction of gsh in the spinal cord and motor neuron cells is correlated with aif translocation caspase 3 activation and motor neuron degeneration during als like disease onset and progression. | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | after permeabilization with 0.2% triton x 100 in pbs and blocking with 10% goat serum cells were incubated with specific antibodies overnight at 4 _amp_#x000b0;c aif cytochrome c and active caspase 3 antibodies were all at 1:300 dilution; chemicon inc. . | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | results gsh depletion promoted aif redistribution cytochrome c release caspase 3 activation and apoptotic cell death a biochemical assay and an immunohistochemical analysis were used to identify the molecular pathways of cell apoptosis by gsh depletion. | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | gsh depletion increased caspases 3 activation revealed by immunohistochemical staining with anti active caspase 3 antibody figure 7a_amp_#x02013;7c . | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | decreased gsh was associated with aif nuclear translocation caspase 3 activation and motor neuron cell death figure 9 in als like transgenic mice. | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | cytochrome c mediated caspase 3 activation elicited by gsh reduction also contributed to motor neuron cell death apoptosis . | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | the dramatic increase in cytochrome c release to cytosol detected by the fractionation assay and immunohistochemical analysis figure 6b and 6d most likely reflects the increased activation of caspase 3 and consequently contributes to the markedly increased of apoptosis elicited through the reduction of gsh in nsc34 cells figure 7 . | | |
| 17150307 | caspase 3 | caspase 3 | 1.0 | figure 9 redistribution of aif and active caspase 3 in motor neuron cells of als like mice | | |
| 17496232 | caspase 3 | caspase 3 | 1.0 | elease of cytochrome c triggering the assembly of the apoptosome complex with apoptotic protease activating factor 1 as central scaffold protein that directly recruits caspase 9 which in turn elicits caspase 3 effects 31 . | | |
| 17496232 | caspase 3 | caspase 3 | 1.0 | no donors or endogenous no induces apoptotic cell death with the activation of jnk/sapk and p38 mapk and caspase 3 or inactivation of nf kappab. | | |