HUGO ID Detailed Result 3711

PMID	Match String	Actual String	Score	Flanking text	Edited by	Edit
10077670	rotamase	rotamase	1.0	because both groups of drugs inhibit the rotamase activity of cyclophilins cyp but only the immunosuppressant analogs inhibit calcineurin activity these data suggest that rotamase inhibition underlies the enhanced cell death after sodv148g expression.
10077670	rotamase	rotamase	1.0	the importance of rotamase activity in mutant sod mediated apoptosis was supported by experiments showing that overexpressed wild type cyclophilin a cypa but not cypa with a rotamase active site point mutation protected cells from death after sodv148g expression.
10077670	rotamase	rotamase	1.0	these data suggest that mutant sod produces a greater need for rotamase and also highlights possible new therapeutic strategies in fals.
10077670	rotamase	rotamase	1.0	rotamase activity assay.
10077670	rotamase	rotamase	1.0	a previously published 21 chymotrypsin coupled assay was used to measure rotamase activity.
10077670	rotamase	rotamase	1.0	cells were treated with csa and were harvested as described above for the rotamase assay.
10077670	rotamase	rotamase	1.0	fig. 3 a shows that csa csg and fk506 immunosuppressant drugs known to inhibit calcineurin and the rotamase activity of immunophilin potentiated cell death induced by sodv148g expression fig 3 a .
10077670	rotamase	rotamase	1.0	we also examined the effect of pkf 211_amp_#x02013;811 and psc 833 nonimmunosuppressant csa analogs that inhibit cyclophilin rotamase activity but not calcineurin 22 23 .
10077670	rotamase	rotamase	1.0	the results suggest that csa enhances sodv148g induced cell death by inhibiting cyclophilin rotamase activity and not because of calcineurin inhibition.
10077670	rotamase	rotamase	1.0	effect of csa on rotamase activity levels.
10077670	rotamase	rotamase	1.0	the above studies suggested that csa and its immunosuppressive analogs might enhance sod mutant induced cell death through its interference with rotamase activity.
10077670	rotamase	rotamase	1.0	it also raised the question as to whether there is a preexisting difference in the level of rotamase activity between sodv148g expressing and mock cells making the former cells more sensitive to csa.
10077670	rotamase	rotamase	1.0	therefore we measured rotamase activity in mock wt and mutant sod expressing cells before and after csa treatment.
10077670	rotamase	rotamase	1.0	the data show that all cells had a similar amount of rotamase activity before csa treatment and that csa decreased rotamase activity to a similar level in all three groups table 1 with no significant differences in this inhibition in the sodv148g expressing cells vs. other csa treated groups.
10077670	rotamase	rotamase	1.0	these results suggested that mutant sod expressing cells might be more sensitive to effects of decreased rotamase activity.
10077670	rotamase	rotamase	1.0	our interpretation of these studies is that human sod/rodent sod heterodimers that are formed in the transiently expressing cells may be more sensitive to conformational changes resulting from rotamase inhibition than the homogeneous rodent homodimers present in controls.
10077670	rotamase	rotamase	1.0	to further examine the role of cyclophilin rotamase activity on mutant sod induced cell death we transfected ngf differentiated pc12 cells with wild type cypa cypawt cdna or cypa r55a cdna that contains a point mutation in the putative rotamase active
10077670	rotamase	rotamase	1.0	activity on mutant sod induced cell death we transfected ngf differentiated pc12 cells with wild type cypa cypawt cdna or cypa r55a cdna that contains a point mutation in the putative rotamase active site.
10077670	rotamase	rotamase	1.0	these results suggest that the rotamase function of cypa protects cells from death induced by sodv148g.
10077670	rotamase	rotamase	1.0	these results suggest that modulating the rotamase activity is vital for the survival of mutant sod expressing cells.
10077670	rotamase	rotamase	1.0	in addition to inhibiting calcineurin and blocking the mptp csa blocks peptidyl prolyl isomerase rotamase activity of cyclophilins.
10077670	rotamase	rotamase	1.0	immunophilin rotamase activity is believed to have a variety of actions in neurons 15 .
10077670	rotamase	rotamase	1.0	to test whether csa enhanced sodv148g toxicity through rotamase inhibition we examined the effect of immunosuppressants csa csg and fk506 as well as nonimmunosuppressants pkf 211_amp_#x02013;811 or psc 833 .
10077670	rotamase	rotamase	1.0	nonimmunosuppressants do not influence calcineurin activity but do inhibit rotamase activity.
10077670	rotamase	rotamase	1.0	we found that both drug classes enhanced apoptosis induced by mutant sod indicating that the action of csa may be related to rotamase inhibition and not to calcineurin inhibition.
10077670	rotamase	rotamase	1.0	a role for rotamase in mutant sod toxicity was supported by experiments comparing mutant sod induced cell death after expression of cypawt vs. an isomerase activity deficient mutant cypa r55a .
10077670	rotamase	rotamase	1.0	although a recent report concluded that cypa r55a may have more rotamase activity than previously suspected 33 34 it appears that this residual activity is insufficient to permit folding of fully functional proteins 17 .
10077670	rotamase	rotamase	1.0	our data highlight the importance of rotamase activity in modifying the effect of mutant sod.
10077670	rotamase	rotamase	1.0	the findings that rotamase activity protects cells from mutant sod induced cell death and that there is roughly similar rotamase activity after csa treatment in wt and mutant sod expressing cells suggests that cells expressing mutant sod have a greater reliance on rotamase activity and has implications on our understanding of
10077670	rotamase	rotamase	1.0	activity after csa treatment in wt and mutant sod expressing cells suggests that cells expressing mutant sod have a greater reliance on rotamase activity and has implications on our understanding of the mechanism of this toxicity.
10077670	rotamase	rotamase	1.0	the increased protein turnover may enhance the need for rotamase function.
10077670	rotamase	rotamase	1.0	rotamase also may be needed to refold proteins that are partly denatured by oxidative damage.
10077670	rotamase	rotamase	1.0	rotamase may be required to maintain proteins in a correct conformation during their transport in motor axons.
10077670	rotamase	rotamase	1.0	rotamase also may have a role in the normal folding of sod itself to help stabilize the enzyme or its dimers.
10077670	rotamase	rotamase	1.0	mutant sod expression than after sodwt expression suggesting that the mutant enzyme was more sensitive than wt to the effects of csa perhaps because the mutant sod was more sensitive to a decline in rotamase activity and the consequences of misfolding.
10077670	rotamase	rotamase	1.0	it also may be that other proteins perhaps ones that interact with sod are more sensitive to decreased rotamase activity.
10077670	rotamase	rotamase	1.0	our observations have implications regarding possible therapy of fals suggesting the potential use of chaperone proteins or of agents that modulate rotamase activity to correct the deficits.
10077670	rotamase	rotamase	1.0	rather our studies suggest that these drugs worsen the fals mediated cell death because of rotamase inhibition.
10077670	rotamase	rotamase	1.0	table 1 mean rotamase and sod activity _amp_#x0005b;percent of control mock infected pc12 cells_amp_#x0005d;
10077670	rotamase	rotamase	1.0	suppressants bind immunophilins _amp_#x0005b;the drug receptor: e.g. cyclophilins cyp or fk binding protein_amp_#x0005d; and the drug immunophilin complex inhibits calcineurin activity as well as the rotamase peptidyl prolyl cis_amp_#x02013;trans isomerase activity of the immunophilins 13 14 .
10077670	rotamase	rotamase	1.0	rotamase facilitates cis_amp_#x02013;trans isomerization of the peptide bond on the n terminal side of proline residues aids normal folding and assembly of proteins including sod and has other cellular functi
10077670	rotamase	rotamase	1.0	most importantly we show that immunosuppressant drugs csa csg and fk506 enhance cell death induced by mutant sod and that this enhancement depends on rotamase inhibition.
10077670	rotamase	rotamase	1.0	these findings suggest that mutant sod may lead to increased protein damage or turnover and a greater reliance on rotamase activity.