HUGO ID Detailed Result 1504

HUGO ID 1504
Symbol CASP3
Name caspase 3, apoptosis-related cysteine peptidase
#Occurrence 52
#Paper 14

 

PMID Match String Actual String Score Flanking text Edited by Edit
11173059caspase 3caspase 31.0e of dna fragmentation a biochemical marker of apoptosis the altered expression of mrna for bcl 2 and bax an anti apoptotic and a proapoptotic gene respectively and the demonstration of caspase 1 and caspase 3 activation which are specific intracellular proteases responsible for the execution of the cell death program yoshiyama ; martin and li .  
11173059caspase 3caspase 31.0the apoptosis effector molecule caspase 3 and the anti apoptotic molecule bcl 2 are overexpressed in the basal ganglia of patients with parkinson's disease confirming that apoptosis is a relevant mechanism of neural death in this paradigm ma 
12060810caspase 3caspase 31.0second minocycline inhibits caspase 1 and caspase 3 expression decreases inos activity and delays mortality in a transgenic mouse model of huntington_amp_#39;s disease _amp_#91; 5 _amp_#93;.  
12843244caspase 3caspase 31.0caspase 11 is a key regulator of caspase 1 and caspase 3 activation under pathological conditions.  
12843244caspase 3caspase 31.0the caspase 1 and caspase 3 like activities as well as the level of interleukin 1 beta were significantly reduced in the spinal cord of symptomatic caspase 11 / ;sod1 g93a mice compared with that of caspase 11 +/ ; sod1 g93a mi 
12843244caspase 3caspase 31.0activation of caspase 1 and caspase 3 in the spinal cords of mutant sod1 mice and als patients has been reported previously martin 1999 ; li et al. 2000 ; pasinelli et al. 2000 ; vukosavic et al. 2000 .  
12843244caspase 3caspase 31.0the possible involvement of both caspase 1 and caspase 3 in the pathogenesis of als strongly suggested the involvement of caspase 11 in this process because caspase 11 is a key upstream regulator of both caspase 1 and 3 under pathological conditions wang e 
12843244caspase 3caspase 31.0apoptosis and caspase 3 activation by lps shock or ischemic brain injury are also defective in caspase 11 / mice kang et al. 2000 2002 .  
12843244caspase 3caspase 31.0activation of caspase 1 and caspase 3 in the spinal cord of presymptomatic g93a mice has been reported previously li et al. 2000 ~60 d of age consistent with reports of active caspase 1 being present in the spinal cords of g93a mice li e 
12843244caspase 3caspase 31.0low levels of active caspase 3 were first detected at day 60 in g93a mice at approximately the time of caspase 11 induction.  
12843244caspase 3caspase 31.0because the lack of caspase 11 resulted in the significant inhibition of caspase 1 and caspase 3 activation our results suggest that caspase 1 and caspase 3 activation is not crucial for neurodegeneration in this mouse model of als.  
12843244caspase 3caspase 31.0our study cannot rule out however that the residual caspase activity in caspase 11 / background sufficient for neurodegeneration and caspases other than caspase 1 and caspase 3 may be involved in mediating neuronal degeneration in g93a mice.  
12843244caspase 3caspase 31.0upregulation of caspase 11 at the disease onset in g93a mice and reduction of caspase 1 and caspase 3 activation in caspase 11 / ;g93a double mutant mice.  
12843244caspase 3caspase 31.0a upregulation of caspase 11 and appearance of active caspase 1 and caspase 3 fragments in the g93a spinal cords.  
12843244caspase 3caspase 31.0eighty micrograms of the samples were also immunoblotted with monoclonal anti caspase 1 antibody for detection of full length caspase 1 c1fl and the active form active c1 and with anti active caspase 3 antibody active c3 . *igg h igg heavy chain; *nsp nonspecific.  
12843244caspase 3caspase 31.0the caspase 3 caspase 1 and caspase 11 like enzyme activities were measured using fluorogenic substrates acdevd amc devd acyvad amc yvad and acvehd amc vehd respectively in the lysates n = 4; mean _amp_#177; sd .  
12843244caspase 3caspase 31.0consistent with the activation of caspase 1 and caspase 3 and the caspase 11 induction profile by immunoblot fig 1 a acyvad amc caspase 1 like acdevd amc caspase 3 like and acvehd amc caspase 11 like cleavage activities thornberry et al. 1997 ; kang et al. 2000 were elevated in the lumbar and thoracic spinal cords of g93a mice fig 1 b .  
12843244caspase 3caspase 31.0in caspase 11 / ; g93a mice the absence of caspase 11 resulted in a significant reduction of caspase 3 and caspase 1 like activities as well as caspase 11 like activity fig 1 b .  
15081582caspase 3caspase 31.0in rat cortical cells high concentrations of pge 2 induce apoptosis in a dose dependent manner likely via the ep2 receptor and subsequent activation of caspase 3 takadera et al. 2002 .  
15081582caspase 3caspase 31.0exposure to pgd 2 synthase induces apoptosis in pc12 neuronal cells via caspase 3 activation.  
15210305caspase 3caspase 31.0ough morphological criteria and gene expression analysis of specific apoptosis related gene candidates such as bcl xl and the calcineurin mediated bad dephosphorylation which is known to activate the caspase 3 apoptotic cascade [ 103 ].  
15571972caspase 3caspase 31.0this was accompanied by a substantial reduction of caspase 3 activity inflammation and gliosis kriz et al. 2003 .  
15572176caspase 3caspase 31.0some motor neurons plated on these reactive astrocytes were smaller and displayed immunoreactivity for cleaved caspase 3 suggesting the activation of apoptotic mechanisms.  
16624536caspase 3caspase 31.0an in vitro experiment demonstrated in mixed primary spinal cord cultures that igg from patients with als induces apoptosis via the caspase 3 pathway selectively in motor neurons [107] .  
16647138caspase 3caspase 31.0pge 2 one of its products is implicated in inflammation and the apoptosis of cortical cells by acting on the ep2 receptor which in turn activates caspase 3 a pro apoptotic agent takadera et al. 2002 .  
16647138caspase 3caspase 31.0these inhibitors also prevent the prp106_amp_#x2013;126 induced caspase 3 activation and annexin v binding involved in 5 lox mediated apoptosis.  
16737688caspase 3caspase 31.0primary antibodies were used to further evaluate neurodegenerative alterations sod1 and tyrosine hydroxylase to identify the molecular changes associated with degeneration caspase 3 active and to characterize the immune reaction.  
16737688caspase 3caspase 31.0fects neurons as well as glial cells and neurites before somata v spreads caudocranially from the spinal cord up to telencephalic areas and vi does not involve the activation of the apoptotic protein caspase 3.  
16737688caspase 3caspase 31.0in addition we performed immunohistochemical stainings of active caspase 3.  
16737688caspase 3caspase 31.0we did not find evidence for the expression of casp3act in any animal suggesting that either caspase 3 is not involved in cell death and consequently that apoptosis is not a major cell death mechanism in the sod1 g93a transgenics.  
17015226caspase 3caspase 31.0a final apoptotic mechanism is probably a central aspect of neuronal death as activation of the executioner caspase 3 is found in mouse models contemporaneous with neuronal death li et_amp_#xa0;al. 2000 and pasinelli et_amp_#xa0;al. 2000 and lowered levels of the antiapoptotic bcl 2 have been reported in spinal cord 
17015226caspase 3caspase 31.0intriguingly it has recently been demonstrated that caspase 3 activation in glial cells proteolytically inactivates the glutamate transporter eaat2 boston howes et_amp_#xa0;al. 2006 .  
17114826caspase 3caspase 31.0apoptotic neurons were identified in aluminum injected animals that showed significantly increased activated caspase 3 labeling in lumbar spinal cord 255% and primary motor cortex 192% compared with the controls.  
17997855caspase 3caspase 31.0methods hemalum staining cd68 and activated caspase 3 immunocytochemistry and light green staining pneumolysin stimulated sh sy5y cell mono cultures wt sod1 and g93a sod1 were plated on glass coverslips and were fixated with 4% formaldehyde dehydrated t 
17997855caspase 3caspase 31.0cd68 and light green staining was used to distinguish between human macrophages and the sh sy5y cells in co culture and to visualize the morphology of the cells; immunostaining for activated caspase 3 was used to detect apoptosis in pneumolysin treated g93a sod1 cells: fixated cells were permeabilised with triton x 0.1% in pbs for 30 minutes and then incubated with cd68 antibody clone kp1 dilution 
17997855caspase 3caspase 31.0sin treated g93a sod1 cells: fixated cells were permeabilised with triton x 0.1% in pbs for 30 minutes and then incubated with cd68 antibody clone kp1 dilution 1:50 dako glostrup denmark or activated caspase 3 antibody [rabbit anti caspase 3 cleaved dilution 1:100 kindly donated by zytomed systems berlin germany] for 90 minutes.  
17997855caspase 3caspase 31.0 antibody [rabbit anti caspase 3 cleaved dilution 1:100 kindly donated by zytomed systems berlin germany] for 90 minutes.  
17997855caspase 3caspase 31.0biotin complex abc vector burlingame ca for 30 min and diaminobenzidine was used for visualisation 5 minutes resulting in a brown staining of the somata of macrophages cd68 /apoptotic g93a sod1 cells caspase 3 .  
17997855caspase 3caspase 31.0sh sy5y cells were counterstained by light green sf yellowish solution chroma gesellschaft schmidt _amp_#x00026; co stuttgart germany after cd68 immunocytochemistry and with hemalum after caspase 3 immunocytochemistry for 1_amp_#x02013;2 minutes rinsed in water dehydrated and mounted with depex serva heidelberg germany .  
17997855caspase 3caspase 31.0by in situ tailing morphology arrows fig 3 and by immunocytochemistry for activated caspase 3 fig 4 of g93a sod1 neuroblastoma cells it became apparent that a large proportion of these cells died by apoptosis.  
17997855caspase 3caspase 31.0apoptotic g93a sod1 sh sy5y cells after incubation with 0.5 _amp_#x003bc;g/ml ply for three hours detected by immunocytochemistry for activated caspase 3.  
18436268caspase 3caspase 31.0moreover fa or fa + b12 treatment decreased the levels of cleaved caspase 3 and poly adp ribose polymerase parp but up regulated the level of anti apoptotic protein bcl 2.  
18436268caspase 3caspase 31.0 _amp_#x3bc;m pvdf membrane incubated with primary antibodies of inos 1:5000 chemicon ca usa tnf _amp_#x3b1; 1:1000 cell signaling ma usa bcl 2 1:1000 santa cruz biotechnology inc. santa cruz cleaved caspase 3 1:1000 cell signaling ma usa parp and cleavage 1:1000 cell signaling ma usa respectively.  
18436268caspase 3caspase 31.0to investigate whether hcy lowering drugs fa and b12 had the anti apoptotic effects in the sod1 g93a transgenic mice we determined the protein level of bcl 2 cleaved caspase 3 and parp in the spinal cord after fa b12 or fa + b12 treatment.  
18436268caspase 3caspase 31.0we found that fa or fa + b12 treatment can increase the level of bcl 2 and reduce the level of cleaved caspase 3 and cleaved parp fig._amp_#xa0;5 b .  
18436268caspase 3caspase 31.0moreover we demonstrated that fa or fa + b12 treatment has significant anti apoptotic effects by increasing bcl 2 expression and inhibiting cleaved caspase 3 and cleaved parp.  
18436268caspase 3caspase 31.0hcy could induce apoptosis by regulating the expression of several important proteins such as bcl 2 cleaved caspase 3 and cleaved parp [baydas et_amp_#xa0;al. 2005] and [kruman et_amp_#xa0;al. 2000] .  
18436268caspase 3caspase 31.0cleaved caspase 3 and cleaved parp represent the downstream signals of apoptosis which may contribute to the motor neuron death in sod1 g93a mice pasinelli et al. 2000 .  
18436268caspase 3caspase 31.0r study suggested that fa or fa + b12 treatment could up regulate the expression of anti apoptotic protein bcl 2 as well as down regulated the expression of apoptosis related proteins such as cleaved caspase 3 and cleaved parp.  
18436268caspase 3caspase 31.0moreover we found that fa or fa + b12 treatment can significantly inhibit the levels of cleaved caspase 3 and cleaved parp as well as up regulate the levels of bcl 2.  
18436268caspase 3caspase 31.0 b western blot of bcl 2 cleaved caspase 3 and cleaved parp from spinal cord samples.  
18436268caspase 3caspase 31.0 c_amp_#x2013;g quantitative data of the expression of inos tnf _amp_#x3b1; bcl 2 cleaved caspase 3 and cleaved parp in five groups.