)| PMID |
18308427 ( ) |
|---|---|
| Title | Oxidant-antioxidant imbalance in the erythrocytes of sporadic amyotrophic lateral sclerosis patients correlates with the progression of disease. |
| Abstract | Free radicals are implicated in numerous disease processes including motor neuron degeneration (MND). Antioxidant defense enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G-6-PDH) in the erythrocytes are capable of detoxifying reactive oxygen species produced endogenously or exogenously. In the present study, the extent of lipid peroxidation (LPO) and antioxidant defenses were evaluated in the erythrocytes of 20 sporadic amyotrophic lateral sclerosis (ALS) patients and 20 controls. We observed that lipid peroxidation in the erythrocytes of amyotrophic lateral sclerosis patients significantly increased with respect to controls (P<0.001). On the other hand, catalase activity was found to be significantly lower (P<0.001). The activities of glucose-6-phosphate dehydrogenase, glutathione reductase and glutathione levels were also found to be significantly reduced in ALS patients compared to healthy subjects (P<0.001, P<0.01 and P<0.01, respectively). It was further observed that lipid peroxidation started to increase and catalase, glutathione reductase, glucose-6-phosphate dehydrogenase enzyme activities and glutathione levels started to decrease as amyotrophic lateral sclerosis progressed from 6 to 24 months, suggesting a correlation between these parameters and duration of amyotrophic lateral sclerosis. This study confirms the involvement of oxidative stress during the progression of amyotrophic lateral sclerosis and the need to develop specific peripheral biomarkers. Sciences, Raebareli Road, Lucknow 226014, UP, India. gnageshbabu@yahoo.com <gnageshbabu@yahoo.com> |
NOTE: Color highlight is limited to the abstract and SciMiner text-mining mode. If you see much more identified targets below from "Targets by SciMiner Summary" and "Targets by SciMiner Full list", they may have been identified from the full text.
Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 1516 | CAT | catalase | 13 | CAT | catalase | |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | 9 | SOD | superoxide dismutase | |
| 4623 | GSR | glutathione reductase | 7 | glutathione reductase | |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | 6 | glucose 6 phosphate dehydrogenase | |
| 399 | ALB | albumin | 1 | serum albumin | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | Antioxidant defense enzymes superoxide dismutase (SOD), SOD catalase (CAT), CAT glutathione peroxidase (GSHPx), GSHPx glutathione reductase (GR) |
| 1516 | CAT | catalase | CAT | 1.0 | Antioxidant defense enzymes superoxide dismutase (SOD), SOD catalase (CAT), CAT glutathione peroxidase (GSHPx), GSHPx glutathione reductase (GR) GR and glucose-6-phosphate |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | These enzymes include superoxide dismutase (SOD) SOD (E.C E.C No 1.15.1.1 that remove O 2_amp_#x2212 by catalyzing |
| 1516 | CAT | catalase | CAT | 1.0 | Catalase (CAT) CAT (E.C E.C No 1.11.1.6 catalyzes the conversion of hydrogen peroxide |
| 1516 | CAT | catalase | CAT | 1.0 | other sulfydryl groups of a cell and the antioxidant enzyme CAT in its active form ( Kletzien et al. 1994 and |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | the correlation if any between the lipid peroxidation (LPO), LPO SOD CAT GSH GSHPx GR and G-6-PDH levels and the progression |
| 1516 | CAT | catalase | CAT | 1.0 | correlation if any between the lipid peroxidation (LPO), LPO SOD CAT GSH GSHPx GR and G-6-PDH levels and the progression of |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | SOD activity was estimated by the method of Nishikimi et al |
| 1516 | CAT | catalase | CAT | 1.0 | CAT was estimated by the method of Aebi (1984) 1984 |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | However the activities of SOD and GSHPx were found to be insignificantly changed in comparison |
| 1516 | CAT | catalase | CAT | 1.0 | found that LPO ( Fig 6 started to increase and CAT GSH GR and G-6-PDH levels ( Fig 7 Fig 8 |
| 1516 | CAT | catalase | CAT | 1.0 | In the present study CAT was found to exhibit significantly reduced activity when compared to |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 1.7 | elevated blood free radical levels did not induce the erythrocyte SOD and GSHPx enzyme activities in sporadic amyotrophic lateral sclerosis patients |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | antioxidant defense enzymes: superoxide dismutase sod catalase cat glutathione peroxidase gshpx glutathione reductase gr and glucose 6 phosphate dehydrogenase g 6 pdh in the erythrocytes are capable of detoxifying reactive oxygen species produced endogenously or exogenously. |
| 1516 | CAT | catalase | catalase | 1.0 | antioxidant defense enzymes: superoxide dismutase sod catalase cat glutathione peroxidase gshpx glutathione reductase gr and glucose 6 phosphate dehydrogenase g 6 pdh in the erythrocytes are capable of detoxifying reactive oxygen species produced endogenously or |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | superoxide dismutase | 1.0 | antioxidant defense enzymes: superoxide dismutase sod catalase cat glutathione peroxidase gshpx glutathione reductase gr and glucose 6 phosphate dehydrogenase g 6 pdh in the erythrocytes are capable of detoxifying reactive oxygen species produced en |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | antioxidant defense enzymes: superoxide dismutase sod catalase cat glutathione peroxidase gshpx glutathione reductase gr and glucose 6 phosphate dehydrogenase g 6 pdh in the erythrocytes are capable of detoxifying reactive oxygen species produced endogenously or exogenously. |
| 1516 | CAT | catalase | catalase | 1.0 | on the other hand catalase activity was found to be significantly lower p _amp_#x3c; 0.001 . |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | the activities of glucose 6 phosphate dehydrogenase glutathione reductase and glutathione levels were also found to be significantly reduced in als patients compared to healthy subjects p _amp_#x3c; 0.001 p _amp_#x3c; 0.01 and p _amp_#x3c; 0.01 respectively . |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | the activities of glucose 6 phosphate dehydrogenase glutathione reductase and glutathione levels were also found to be significantly reduced in als patients compared to healthy subjects p _amp_#x3c; 0.001 p _amp_#x3c; 0.01 and p _amp_#x3c; 0.01 respec |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | it was further observed that lipid peroxidation started to increase and catalase glutathione reductase glucose 6 phosphate dehydrogenase enzyme activities and glutathione levels started to decrease as amyotrophic lateral sclerosis progressed from 6 to 24 months suggesting a correlation between these p |
| 1516 | CAT | catalase | catalase | 1.0 | it was further observed that lipid peroxidation started to increase and catalase glutathione reductase glucose 6 phosphate dehydrogenase enzyme activities and glutathione levels started to decrease as amyotrophic lateral sclerosis progressed from 6 to 24 months suggesting a corre |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | it was further observed that lipid peroxidation started to increase and catalase glutathione reductase glucose 6 phosphate dehydrogenase enzyme activities and glutathione levels started to decrease as amyotrophic lateral sclerosis progressed from 6 to 24 months suggesting a correlation between these parameters and duration of amyotrop |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | superoxide dismutase | 1.0 | these enzymes include superoxide dismutase sod e.c no 1.15.1.1 that remove o 2_amp_#x2212; by catalyzing its dismutation one o 2_amp_#x2212; being reduced to h 2 o 2 and another oxidized to o 2 [fridovich 1989] [halliwell 2001] and [liochev a |
| 1516 | CAT | catalase | catalase | 1.0 | catalase cat e.c no 1.11.1.6 catalyzes the conversion of hydrogen peroxide h 2 o 2 to water and molecular oxygen thereby protecting cells from the toxic effects of hydrogen peroxide. |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | gsh recycling is catalyzed by glutathione reductase gr e.c no 1.8.1.10 which uses reducing equivalents from nadph to reconvert gssg to 2gsh. |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | low erythrocyte gsh is also manifest in hereditary nonspherocytic lymphocytic leukemia and glucose 6 phosphate dehydrogenase g 6 pdh e.c no 1.1.1.49 deficiency. |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | superoxide dismutase | 1.0 | superoxide dismutase |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | the 1 ml reaction mixture contained tris_amp_#x2013;hcl buffer 25 mm ph 7.6; nadph 0.12 mm; glutathione reductase 1 u/ml; reduced glutathione 1 mm; cumin hydroperoxide 0.1 mm and suitable amount of enzyme. |
| 399 | ALB | albumin | serum albumin | 1.0 | y using the method of brehe and burch 1976 with minor modifications. 200 _amp_#x3bc;l of 0.01n hcl was mixed with dtnb containing buffer 110 mm na 2 hpo 4 40 mm nah 2 po 4 15 mm edta 0.04% w/v bovine serum albumin bsa and 0.3 mm dtnb. |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | glutathione reductase |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | glucose 6 phosphate dehydrogenase |
| 1516 | CAT | catalase | catalase | 1.0 | the rate of lpo in the erythrocytes of als patients was significantly increased with respect to control subjects p _amp_#x3c; 0.001 fig 1 whereas catalase activity in the rbc of als patients was significantly lower than the controls p _amp_#x3c; 0.01 fig 2 . |
| 1516 | CAT | catalase | catalase | 1.0 | fig. 7._amp_#xa0;second degree polynomial regression plot showing the activity of catalase in the erythrocytes of als vs. duration of illness n = 10 at 6 months 5 at 12 months and 5 at 24 months . |
| 4623 | GSR | glutathione reductase | glutathione reductase | 1.0 | fig. 9._amp_#xa0;second degree polynomial regression plot showing the activity of glutathione reductase vs. duration of illness in the erythrocytes of patients with als n = 10 at 6 months 5 at 12 months and 5 at 24 months . |
| 4057 | G6PD | glucose-6-phosphate dehydrogenase | glucose 6 phosphate dehydrogenase | 1.0 | fig. 10._amp_#xa0;second degree polynomial regression plot showing the activity of glucose 6 phosphate dehydrogenase vs. duration of illness in the erythrocytes of als patients n = 10 at 6 months 5 at 12 months and 5 at 24 months . |