HUGO ID Detailed Result 576

HUGO ID 576
Symbol APAF1
Name apoptotic peptidase activating factor 1
#Occurrence 50
#Paper 3

 

PMID Match String Actual String Score Flanking text Edited by Edit
14739060APAF1Apaf10.3Cytochrome c and the cytosolic factor Apaf1 activate the caspases while apoptosis-initiating factor and caspase activated DNase 
16046141APAF1Apaf12.1dismutases (SOD1) SOD1 typical of familial ALS is mediated by Apaf1 a scaffold protein involved in neural development 
16046141APAF1Apaf12.1origin and modulating the expression of both mutant SOD1s and Apaf1 we show that the removal of Apaf1 prevents cells death 
16046141APAF1Apaf12.1mutant SOD1s and Apaf1 we show that the removal of Apaf1 prevents cells death 
16046141APAF1Apaf12.1c becomes part of the apoptosome a complex in which Apaf1 serves as a scaffold protein also for the binding of 
16046141APAF1Apaf12.1In this study we have investigated on the role of Apaf1 and the apoptosome in the induction of death in cell 
16046141APAF1Apaf12.1Our data demonstrate that removal of Apaf1 prevents cell death and mitochondrial damage by intercepting activation of 
16046141APAF1Apaf12.1Apaf1 expression plasmid was created by cloning of Apaf1 cDNA ( 
16046141APAF1Apaf12.1Apaf1 expression plasmid was created by cloning of Apaf1 cDNA ( Cecconi et al. 1998 under control of the 
16046141APAF1Apaf12.1Embryonic Telencefalic Naive Apaf1 (ETNA_amp_#x2212;/_amp_#x2212;) ETNA_amp_#x2212 _amp_#x2212 cell lines were obtained from e14 embryos 
16046141APAF1Apaf12.1ETNA_amp_#x2212 _amp_#x2212 cell lines were obtained from e14 embryos of Apaf1 knock-out mice as previously described ( Cozzolino et al. 2004 
16046141APAF1Apaf12.1Apaf1 was detected with a rat mAb anti-Apaf1 (clone clone 18H2 
16046141APAF1Apaf12.1Overexpression of Apaf1 exacerbates apoptotic death induced by fALS-SOD1 in human neuroblastoma 
16046141APAF1Apaf12.1To analyze whether Apaf1 is involved in mutant SOD1-induced apoptosis we stably transfected SH-SY5Y 
16046141APAF1Apaf12.1stably transfected SH-SY5Y neuroblastoma cells with a plasmid coding for Apaf1 
16046141APAF1Apaf12.1C05 C19 and C20 were chosen for equivalent expression of Apaf1 
16046141APAF1Apaf12.1results and data from clone C05 (named named SH/Apaf1) SH Apaf1 are shown 
16046141APAF1Apaf12.1Western blotting with specific anti-Apaf1 antibodies shows that SH/Apaf1 SH Apaf1 neuroblastoma cells express high levels of Apaf1 ( Fig 2 
16046141APAF1Apaf12.1that SH/Apaf1 SH Apaf1 neuroblastoma cells express high levels of Apaf1 ( Fig 2 A 
16046141APAF1Apaf12.1In this conditions no Apaf1 expression is detectable in total cell lysates from control cells 
16046141APAF1Apaf12.1total cell lysates from control cells although SH-SY5Y do express Apaf1 as assessed by us in immunoprecipitation experiments (not not shown 
16046141APAF1Apaf12.1Transient transfection of control SH cells or SH/Apaf1 SH Apaf1 cells with wtSOD1 or G93A-SOD1 mutant elicits high level comparable 
16046141APAF1Apaf12.1of caspase-3 is dramatically increased only in G93A-expressing SH/Apaf1 SH Apaf1 cells as compared to G93A-transfected SH cells ( Fig 2 
16046141APAF1Apaf12.120-fold increase of apoptotic nuclei is observed in SH/Apaf1 SH Apaf1 cells expressing the mutant SOD1 with respect to untransfected control 
16046141APAF1Apaf12.1Deletion of Apaf1 prevents apoptotic death induced by fALS-SOD1 in mouse neuronal cells 
16046141APAF1Apaf12.1order to establish an experimental system where the role of Apaf1 in fALS-SOD1-induced cell death could be thoroughly studied embryonal cortical 
16046141APAF1Apaf12.1thoroughly studied embryonal cortical cells from a control mouse (Apaf1+/+) Apaf1 or a mouse knocked-out for Apaf1 (Apaf1_amp_#x2212;/_amp_#x2212;) Apaf1_amp_#x2212 _amp_#x2212 have 
16046141APAF1Apaf12.1a control mouse (Apaf1+/+) Apaf1 or a mouse knocked-out for Apaf1 (Apaf1_amp_#x2212;/_amp_#x2212;) Apaf1_amp_#x2212 _amp_#x2212 have been isolated and immortalized by the 
16046141APAF1Apaf12.1ETNA_amp_#x2212;/_amp_#x2212; ETNA_amp_#x2212 _amp_#x2212 cells do not express Apaf1 and the absence of this adapter provides them with a 
16046141APAF1Apaf12.1We have observed that Apaf1 deletion also provides these cells with a complete resistance to 
16046141APAF1Apaf12.1Cytochrome c release induced by fALS-SOD1 is independent of Apaf1 expression 
16046141APAF1Apaf12.1expressing different fALS-SOD1s display cytochrome c release independently from the Apaf1 genotype ( Figs 4 B_amp_#x2013 D 
16046141APAF1Apaf1-deficient1.1As shown in Fig 6 A Apaf1-deficient ETNA cells are completely resistant to fALS-SOD1-induced mitochondrial impairment as 
16046141APAF1Apaf12.1On the opposite cotransfection of mutant SOD1s with Apaf1 in ETNA_amp_#x2212;/_amp_#x2212; ETNA_amp_#x2212 _amp_#x2212 cells significantly restores the toxic effect 
16046141APAF1Apaf12.1in these cells is exclusively due to the lack of Apaf1 
16046141APAF1Apaf12.1in ETNA+/+ ETNA cells is significantly reduced in cells lacking Apaf1 ( Fig 6 C 
16046141APAF1Apaf12.1further insight in this process by studying the contribution of Apaf1 to cell death induced by different mutants SOD1s 
16046141APAF1Apaf12.1origin and modulating the expression of both mutant SOD1s and Apaf1 we have observed that indeed Apaf1 is a key factor 
16046141APAF1Apaf12.1both mutant SOD1s and Apaf1 we have observed that indeed Apaf1 is a key factor in the noxious function of fALS-SOD1 
16046141APAF1Apaf12.1factor in the noxious function of fALS-SOD1 while overexpression of Apaf1 exacerbates the induction of apoptosis ( Fig 2 its removal 
16046141APAF1Apaf12.1Fig 2._amp_#xa0 Overexpression of Apaf1 exacerbates apoptotic death induced by fALS-SOD1 in human neuroblastoma 
16046141APAF1Apaf12.1A Na_amp_#xef ve SH-SY5Y cells or SH-SY5Y cells stably overexpressing Apaf1 (SH/Apaf1) SH Apaf1 were transiently transfected with wtSOD1 or with 
16046141APAF1Apaf12.1SH-SY5Y cells or SH-SY5Y cells stably overexpressing Apaf1 (SH/Apaf1) SH Apaf1 were transiently transfected with wtSOD1 or with the G93A-SOD1 mutant 
16046141APAF1Apaf12.1cell lysates was analyzed for the expression of SOD1 and Apaf1 
16046141APAF1Apaf12.1Fig 3._amp_#xa0 Deletion of Apaf1 prevents apoptotic death induced by fALS-SOD1 in mouse neuronal cells 
16046141APAF1Apaf12.14._amp_#xa0 Cytochrome c release induced by fALS-SOD1 is independent of Apaf1 expression 
16046141APAF1Apaf12.1wtSOD1 or G93A G37R G85R and I113T-SOD1 mutants with (Apaf1) Apaf1 or without a plasmid coding for Apaf1 
16046141APAF1Apaf12.1mutants with (Apaf1) Apaf1 or without a plasmid coding for Apaf1 
17496232apoptotic protease activating factor 1apoptotic protease activating factor 11.0no and h 2 o 2 release and activation of intrinsic programmed cell death via apoptotic protease activating factor 1 and activation of p53.  
17496232apoptotic protease activating factor 1apoptotic protease activating factor 11.0in the intrinsic program mitochondrial damage results in the release of cytochrome c triggering the assembly of the apoptosome complex with apoptotic protease activating factor 1 as central scaffold protein that directly recruits caspase 9 which in turn elicits caspase 3 effects 31 .