)| PMID |
12417341 ( ) |
|---|---|
| Title | Differentially expressed genes in sporadic amyotrophic lateral sclerosis spinal cords--screening by molecular indexing and subsequent cDNA microarray analysis. |
| Abstract | To analyze the genes related to the pathophysiology of sporadic amyotrophic lateral sclerosis (SALS) we performed gene profiling of SALS spinal cords using molecular indexing combined with cDNA microarray. Eighty-four fragments were cloned in the first screening procedure with molecular indexing. Subsequent quantitative microarray screening revealed 11 genes which were differentially expressed in SALS. Real-time RT-PCR verified that the expression level of the following six genes was altered in SALS: dorfin, metallothionein-3, 30 kDa TATA-binding protein-associated factor, neugrin, ubiquitin-like protein 5 and macrophage-inhibiting factor-related protein-8. These results indicated that genes associated with the ubiquitin-proteasome system, oxidative toxicity, transcription, neuronal differentiation and inflammation might be involved in the pathogenesis of SALS. Nagoya, Japan. |
NOTE: Color highlight is limited to the abstract and SciMiner text-mining mode. If you see much more identified targets below from "Targets by SciMiner Summary" and "Targets by SciMiner Full list", they may have been identified from the full text.
Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 7408 | MT3 | metallothionein 3 | 10 | MT-3 | metallothionein 3 | |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | 9 | ALS1 | SOD1 | SOD1-mediated | |
| 13432 | RNF19A | ring finger protein 19A | 8 | Dorfin | dorfin | |
| 10417 | RPS27A | ribosomal protein S27a | 4 | ubiquitin | |
| 4141 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | 4 | GAPDH | glyceraldehyde 3 phosphate dehydrogenase | |
| 11543 | TAF10 | TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa | 4 | TAFII30 | |
| 10498 | S100A8 | S100 calcium binding protein A8 | 3 | MRP8 | |
| 10560 | ATXN7 | ataxin 7 | 2 | SCA7 | |
| 8988 | PIN1 | peptidylprolyl cis/trans isomerase, NIMA-interacting 1 | 2 | UBL5 | |
| 4571 | GRIA1 | glutamate receptor, ionotropic, AMPA 1 | 1 | glutamate receptor | |
| 21148 | RNF123 | ring finger protein 123 | 1 | ubiquitin ligase | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | level of the following six genes was altered in SALS dorfin metallothionein-3 30 kDa TATA-binding protein-associated factor neugrin ubiquitin-like protein 5 |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1 | 3.9 | missense mutations in the Cu/Zn Cu Zn superoxide dismutase (SOD1) SOD1 gene 1 while others are considered to be sporadic ALS |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | ALS1 | 3.9 | One SALS case (ALS1) ALS1 and a control (C1) C1 were used for the molecular |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | ALS1 | 3.9 | SALS (ALS1) ALS1 and control (C1) C1 spinal cords were used for molecular |
| 4141 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 1.9 | For internal standard control the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) GAPDH was quantified simultaneously |
| 4141 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 1.9 | probe sequences (forward forward primer reverse primer and TaqMan probe GAPDH 5_amp_#x2032 -CCT GGA GAA ACC TGC CAA GTA T-3_amp_#x2032 5_amp_#x2032 |
| 11543 | TAF10 | TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa | TAFII30 | 2.1 | identical to four known cDNAs 30-kDa TATA-binding protein-associated factor (TAFII30), TAFII30 macrophage-inhibiting factor-related protein-8 (MRP8), MRP8 metallothionein-3 (MT-3) MT-3 and ubiquitin-like |
| 10498 | S100A8 | S100 calcium binding protein A8 | MRP8 | 1.3 | 30-kDa TATA-binding protein-associated factor (TAFII30), TAFII30 macrophage-inhibiting factor-related protein-8 (MRP8), MRP8 metallothionein-3 (MT-3) MT-3 and ubiquitin-like protein 5 (UBL5), UBL5 respectively |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | factor (TAFII30), TAFII30 macrophage-inhibiting factor-related protein-8 (MRP8), MRP8 metallothionein-3 (MT-3) MT-3 and ubiquitin-like protein 5 (UBL5), UBL5 respectively |
| 8988 | PIN1 | peptidylprolyl cis/trans isomerase, NIMA-interacting 1 | UBL5 | 1.0 | (MRP8), MRP8 metallothionein-3 (MT-3) MT-3 and ubiquitin-like protein 5 (UBL5), UBL5 respectively |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | these two unknown genes using RACE methods and named them dorfin and neugrin in previous reports 11 and 12 |
| 11543 | TAF10 | TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa | TAFII30 | 2.1 | TAFII30 is one of the TATA-binding protein-associated factors required for transcription |
| 10560 | ATXN7 | ataxin 7 | SCA7 | 1.0 | An immunohistochemical study using SCA7 transgenic mice showed that TAFII30 is accumulated in nuclear inclusions |
| 11543 | TAF10 | TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa | TAFII30 | 2.1 | An immunohistochemical study using SCA7 transgenic mice showed that TAFII30 is accumulated in nuclear inclusions in neurons |
| 10560 | ATXN7 | ataxin 7 | SCA7 | 1.0 | activity of cellular transcription and contribute to neuronal toxicity in SCA7 model mice 14 |
| 11543 | TAF10 | TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa | TAFII30 | 2.1 | Our result showed up-regulation of the TAFII30 gene in the SALS spinal cords which suggests that this |
| 13432 | RNF19A | ring finger protein 19A | Dorfin | 2.4 | Dorfin is a new RING finger-type ubiquitin ligase containing two RING |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | Our recent study showed that dorfin is localized in the ubiquitinated inclusions of FALS and SALS |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1 | 3.9 | FALS and SALS bound and ubiquitinated various mutant forms of SOD1 in vitro and protected from mutant SOD1-mediated neurotoxicity in mutant |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1-mediated | 3.9 | mutant forms of SOD1 in vitro and protected from mutant SOD1-mediated neurotoxicity in mutant SOD1 culture cells 15 |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1 | 3.9 | in vitro and protected from mutant SOD1-mediated neurotoxicity in mutant SOD1 culture cells 15 |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | These results indicate that dorfin has a crucial role in the pathomechanism of mutant SOD1-mediated |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1-mediated | 3.9 | dorfin has a crucial role in the pathomechanism of mutant SOD1-mediated FALS |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | the present study we showed that the expression of the dorfin gene was significantly higher in the SALS than in the |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | function in FALS and is localized in inclusions of SALS dorfin may play some protective role in the pathogenesis of SALS |
| 13432 | RNF19A | ring finger protein 19A | dorfin | 2.4 | Alzheimer disease Parkinson disease familial spinocerebellar degeneration and ALS 16 dorfin may possibly work to eliminate proteins which are toxic to |
| 10498 | S100A8 | S100 calcium binding protein A8 | MRP8 | 1.3 | MRP8 is mainly expressed in microglial cells in neuronal tissues and |
| 10498 | S100A8 | S100 calcium binding protein A8 | MRP8 | 1.3 | MRP8 decreased in the SALS spinal cords in the present study |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | MT-3 is a neuron-specific member of the zinc- and copper-binding metallothionein |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | The elevation of MT-3 expression in G93A SOD1 transgenic mice was reported 21 and |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1 | 3.9 | The elevation of MT-3 expression in G93A SOD1 transgenic mice was reported 21 and 22 and reduction of |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | transgenic mice was reported 21 and 22 and reduction of MT-3 promotes the onset of disease and death in G93A SOD1 |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD1 | 3.9 | MT-3 promotes the onset of disease and death in G93A SOD1 transgenic mice 23 |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | In sporadic Alzheimer_amp_#x2019 s disease brains MT-3 is significantly down-regulated 19 and 24 |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | In the present study we demonstrated that MT-3 mRNA is decreased in the SALS spinal cords but another |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | another report failed to show a significant difference in the MT-3 mRNA expression level in SALS 25 |
| 7408 | MT3 | metallothionein 3 | MT-3 | 1.3 | The fact that MT-3 was up-regulated in FALS but down-regulated in SALS leads one |
| 8988 | PIN1 | peptidylprolyl cis/trans isomerase, NIMA-interacting 1 | UBL5 | 1.0 | UBL5 is one of the ubiquitin-like proteins |
| 4141 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | GAPDH | 1.9 | shown as the weight ratio of the target gene to GAPDH |
| 7408 | MT3 | metallothionein 3 | metallothionein 3 | 1.0 | real time rt pcr verified that the expression level of the following six genes was altered in sals: dorfin metallothionein 3 30 kda tata binding protein associated factor neugrin ubiquitin like protein 5 and macrophage inhibiting factor related protein 8. |
| 10417 | RPS27A | ribosomal protein S27a | ubiquitin | 1.0 | real time rt pcr verified that the expression level of the following six genes was altered in sals: dorfin metallothionein 3 30 kda tata binding protein associated factor neugrin ubiquitin like protein 5 and macrophage inhibiting factor related protein 8. |
| 4571 | GRIA1 | glutamate receptor, ionotropic, AMPA 1 | glutamate receptor | 1.0 | these include oxidative toxicity glutamate receptor abnormality ubiquitin_amp_#x2013;proteasome dysfunction inflammatory and cytokine activation dysfunction in neurotrophic factors damage to mitochondria cytoskeletal abnormalities and activation of th |
| 4141 | GAPDH | glyceraldehyde-3-phosphate dehydrogenase | glyceraldehyde 3 phosphate dehydrogenase | 1.0 | for internal standard control the expression of glyceraldehyde 3 phosphate dehydrogenase gapdh was quantified simultaneously. |
| 7408 | MT3 | metallothionein 3 | metallothionein 3 | 1.0 | a similarity search revealed that clones 2 7 8 and 11 were identical to four known cdnas 30 kda tata binding protein associated factor tafii30 macrophage inhibiting factor related protein 8 mrp8 metallothionein 3 mt 3 and ubiquitin like protein 5 ubl5 respectively. |
| 10417 | RPS27A | ribosomal protein S27a | ubiquitin | 1.0 | vealed that clones 2 7 8 and 11 were identical to four known cdnas 30 kda tata binding protein associated factor tafii30 macrophage inhibiting factor related protein 8 mrp8 metallothionein 3 mt 3 and ubiquitin like protein 5 ubl5 respectively. |
| 21148 | RNF123 | ring finger protein 123 | ubiquitin ligase | 1.0 | dorfin is a new ring finger type ubiquitin ligase containing two ring finger motifs and an ibr motif at its n terminus. |
| 10417 | RPS27A | ribosomal protein S27a | ubiquitin | 1.0 | ubl5 is one of the ubiquitin like proteins. |
| 10417 | RPS27A | ribosomal protein S27a | ubiquitin | 1.0 | it may possibly organize another ubiquitin like system but its function remains unknown [ 26 ]. |