)| PMID |
19056645 ( ) |
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| Title | Green tea (Camellia sinensis) attenuates nephropathy by downregulating Nox4 NADPH oxidase in diabetic spontaneously hypertensive rats. |
| Abstract | Green tea (GT), through its antioxidant properties, may be useful to treat or prevent human diseases. Because several lines of evidence suggest that oxidative stress contributes to the pathogenesis of diabetic nephropathy, we tested the hypothesis that GT prevents diabetes and hypertension-related renal oxidative stress, attenuating renal injury. Spontaneously hypertensive rats (SHR) with streptozotocin-induced diabetes and nondiabetic SHR were treated daily with tap water or freshly prepared GT (13.3 g/L). After 12 wk, the systolic blood pressure did not differ between treated and untreated nondiabetic or diabetic rats. However, body weight was less (P < 0.05) and glycemia was greater in diabetic SHR rats than in nondiabetic rats. Renal oxidative stress variables such as 8-hydroxy-2'-deoxyguanosine (8-OHdG) and nitrotyrosine expression, NADPH oxidase-dependent superoxide generation, and the expression of renal cortex Nox4 were greater (P < 0.05) in diabetic rats that received water (DW) than in nondiabetic rats that received water (CW). The 8-OHdG and NADPH oxidase-dependent superoxide generation were significantly less in rats treated with GT. Nitrotyrosine and Nox4 expression were significantly less in diabetic rats that received GT (DGT) than in DW. Likewise, the indices of renal injury, albuminuria, and renal expression of collagen IV were significantly greater in DW than in CW. These differences were significantly less in DGT than in DW. GT reestablished the redox state and reduced the indicators of nephropathy without altering glycemia and blood pressure levels in diabetic SHR. These findings suggest that the consumption of GT may ameliorate nephropathy in diabetic hypertensive patients. |
NOTE: Color highlight is limited to the abstract and SciMiner text-mining mode. If you see much more identified targets below from "Targets by SciMiner Summary" and "Targets by SciMiner Full list", they may have been identified from the full text.
Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 7891 | NOX4 | NADPH oxidase 4 | 17 | Nox4 | |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | 12 | nadph oxidase | |
| 2197 | COL1A1 | collagen, type I, alpha 1 | 8 | collagen | |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | 7 | collagen iv | |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | 3 | gp91phox | |
| 399 | ALB | albumin | 3 | albumin | serum albumin | |
| 6081 | INS | insulin | 2 | insulin | |
| 7876 | NOS3 | nitric oxide synthase 3 (endothelial cell) | 1 | endothelial nitric oxide synthase | |
| 11416 | STN | statin | 1 | statin | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | NADPH oxidase-dependent superoxide generation and the expression of renal cortex Nox4 were greater ( P _lt_ 0.05 in diabetic rats that |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | Nitrotyrosine and Nox4 expression were significantly less in diabetic rats that received GT |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | the indices of renal injury albuminuria and renal expression of collagen IV were significantly greater in DW than in CW |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | For instance phosphorothioated antisense oligonucleotides for Nox4 a homolog of the gp91phox subunit of NADPH oxidase inhibits |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 0.0 | instance phosphorothioated antisense oligonucleotides for Nox4 a homolog of the gp91phox subunit of NADPH oxidase inhibits NADPH-dependent ROS generation in the |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | the effects of GT on the expression of NADPH oxidase Nox4 which is a major source of ROS production in the |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | (an an indicator of oxidative/nitrosative oxidative nitrosative stress-induced protein modifications collagen IV and Nox4 (a a homolog of the gp91phox subunit |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | of oxidative/nitrosative oxidative nitrosative stress-induced protein modifications collagen IV and Nox4 (a a homolog of the gp91phox subunit of NADPH oxidase |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 0.0 | modifications collagen IV and Nox4 (a a homolog of the gp91phox subunit of NADPH oxidase by Western blot analysis ( 19 |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | antibody (1:2000; 1 2000 clone 1A6 Upstate goat anti-type IV collagen antibody (1:500; 1 500 Southern Biotech or rabbit polyclonal anti-Nox4 |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | show that GT ameliorates the albuminuria and renal accumulation of collagen IV and reduces markers of renal oxidative stress in diabetic |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | these beneficial effects apparently involves the downregulation of NADPH oxidase Nox4 expression |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | Nox4 a homolog of the gp91phox subunit of NADPH is highly |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 0.0 | Nox4 a homolog of the gp91phox subunit of NADPH is highly expressed in the kidney ( |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | Overexpression of Nox4 in the kidney of diabetic rats with STZ-induced diabetes may |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | ( 20 reported that downregulation of Nox4 induced by antisense oligonucleotides completely attenuated oxidative stress in the |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | mice ameliorated the albuminuria and renal mesangial expansion by downregulating Nox4 |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | In agreement with these findings the downregulation of Nox4 by GT completely attenuated oxidative stress and renal injury in |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | The molecular mechanism by which GT regulates Nox4 activity remains to be elucidated |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | diabetic hypertensive rats by inhibiting oxidative stress through downregulation of Nox4 expression |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | SHR that consumed water or GT for 12 wk of Nox4 ( A and nitrotyrosine ( C |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | Densitometric analysis of both bands was used to determine the Nox4 actin ratio ( B and nitrotyrosine actin ratio ( D |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | SHR that consumed water or GT for 12 wk of collagen IV ( A |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | Densitometric analysis of both bands was used to determine the collagen IV B -tubulin ratio presented as arbitrary units (AU) AU |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | NADPH-dependent superoxide generation Nox4 expression and oxidative stress markers |
| 7891 | NOX4 | NADPH oxidase 4 | Nox4 | 1.7 | The expression of Nox4 was greater ( P = 0.04 in the DW group |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | The urinary AER and the accumulation of kidney collagen IV were used as markers of functional and morphological renal |
| 2197 | COL1A1 | collagen, type I, alpha 1 | collagen | 0.6 | Western blotting showed that renal cortical expression of collagen IV was greater ( P = 0.01 in DW rats |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | renal oxidative stress variables such as 8 hydroxy 2' deoxyguanosine 8 ohdg and nitrotyrosine expression nadph oxidase dependent superoxide generation and the expression of renal cortex nox4 were greater p _lt_ 0.05 in diabetic rats that received water dw than in nondiabetic rats that received water cw . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the 8 ohdg and nadph oxidase dependent superoxide generation were significantly less in rats treated with gt. |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | likewise the indices of renal injury albuminuria and renal expression of collagen iv were significantly greater in dw than in cw. |
| 7876 | NOS3 | nitric oxide synthase 3 (endothelial cell) | endothelial nitric oxide synthase | 1.0 | some of these pathways include enhanced activity of the mitochondrial electron transport chain induced by hyperglycemia 12 increased expression and uncoupling of endothelial nitric oxide synthase leading to greater production of superoxide relative to nitric oxide 9 and activation of the reduced forms of nadph 13 15 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | strategies that reduce superoxide formation via nadph oxidase and/or increase the activity of antioxidant defense systems can attenuate hyperglycemia induced renal injury 18 20 22 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | for instance phosphorothioated antisense oligonucleotides for nox4 a homolog of the gp91phox subunit of nadph oxidase inhibits nadph dependent ros generation in the renal cortex and reduces glomerular hypertrophy and the expression of renal fibronectin 20 . |
| 6081 | INS | insulin | insulin | 1.0 | the administration of epigallocatechin gallate a polyphenol that accounts for ~30% of the dry weight of gt to shr improves endothelial function and insulin sensitivity and reduces blood pressure 24 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | in addition to explore the underlying molecular mechanisms we examined the effects of gt on the expression of nadph oxidase nox4 which is a major source of ros production in the kidney 20 . |
| 6081 | INS | insulin | insulin | 1.0 | diabetic rats received 2 u of long acting insulin novo industry a/s 3 times each week. |
| 399 | ALB | albumin | albumin | 1.0 | body weight plasma glucose systolic blood pressure sbp and albumin excretion rate aer were measured at 0 4 8 and 12 wk. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | nadph oxidase activity was measured by the lucigenin enhanced chemiluminescence method as previously described by gorin et al. |
| 399 | ALB | albumin | serum albumin | 1.0 | protein concentrations were measured using the bradford dye binding method 26 with bovine serum albumin as the standard. |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | renal cortical homogenates were prepared as described elsewhere 19 and were used to quantify nitrotyrosine an indicator of oxidative/nitrosative stress induced protein modifications collagen iv and nox4 a homolog of the gp91phox subunit of nadph oxidase by western blot analysis 19 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | as described elsewhere 19 and were used to quantify nitrotyrosine an indicator of oxidative/nitrosative stress induced protein modifications collagen iv and nox4 a homolog of the gp91phox subunit of nadph oxidase by western blot analysis 19 . |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | the results of this study show that gt ameliorates the albuminuria and renal accumulation of collagen iv and reduces markers of renal oxidative stress in diabetic shr. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the underlying mechanism of these beneficial effects apparently involves the downregulation of nadph oxidase nox4 expression. |
| 11416 | STN | statin | statin | 1.0 | 32 found that the administration of a statin pitavastatin to db/db mice ameliorated the albuminuria and renal mesangial expansion by downregulating nox4. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | figure 1 nadph oxidase activity in renal cortical homogenates from nondiabetic and diabetic shr that consumed water or gt for 12 wk. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | b mean nadph oxidase activities during the last 5 min were used for comparison among the groups. |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | figure 4 representative western blots of the renal cortical expression from nondiabetic and diabetic shr that consumed water or gt for 12 wk of collagen iv a . |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | densitometric analysis of both bands was used to determine the collagen iv: b tubulin ratio presented as arbitrary units au b . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | these results indicated that nadph oxidase was the most likely source of superoxide anion. |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | the urinary aer and the accumulation of kidney collagen iv were used as markers of functional and morphological renal injury respectively. |
| 2202 | COL4A1 | collagen, type IV, alpha 1 | collagen iv | 1.0 | western blotting showed that renal cortical expression of collagen iv was greater p = 0.01 in dw rats than in nondiabetic shr and was reduced to the levels of both nondiabetic groups in the dgt dm x gt; p = 0.03 fig 4 a b . |
| 399 | ALB | albumin | albumin | 1.0 | abbreviations used: 8ohdg 8 hydroxy 2' deoxyguanosine; aer albumin excretion rate; auc area under the curve; cw nondiabetic rats that received water; cgt nondiabetic rats that received daily green tea; dm diabetes mellitus; dw diabetic rats that received water; dgt |