)| PMID |
17951995 ( ) |
|---|---|
| Title | Hypertension increases pro-oxidant generation and decreases antioxidant defense in the kidney in early diabetes. |
| Abstract | AIMS: The combination of hypertension and diabetes exacerbates renal oxidative stress. The aim of the present study was therefore to evaluate the pro-oxidant and antioxidant mechanisms responsible for the induction of renal oxidative stress in the presence of hypertension and diabetes mellitus. METHODS: Diabetes was induced in spontaneously hypertensive rats (SHR) and their genetically normotensive control Wistar-Kyoto (WKY) rats by streptozotocin at 12 weeks of age. After 10 days, pro-oxidant, antioxidant and oxidative stress parameters were evaluated in the renal tissue. RESULTS: NADPH oxidase-dependent superoxide generation in the renal cortex was significantly elevated in WKY and SHR diabetic (D) groups compared to the respective control (C) groups (p < 0.005, n = 5). However, the highest level of superoxide generation was observed in the SHR-D group compared to all other groups. The expression of the gp91phox subunit of NADPH oxidase was significantly elevated in the SHR-D (p < 0.05, n = 5), but not in the WKY-D group, compared to the respective control groups. The renal cortical extracellular-superoxide dismutase level was found to be markedly decreased in the SHR groups compared to the WKY groups (p < 0.05, n = 5). The antioxidant glutathione level was found to be lower in the SHR-D (p = 0.03, n = 15), but not in the WKY-D group, compared to the respective control groups. Finally, nitrotyrosine and 8-hydroxy-2'-deoxyguanosine, markers of oxidative stress, were found to be similar in the kidneys of WKY-C and WKY-D, but were elevated in the SHR-D compared to the SHR-C group. CONCLUSION: We therefore conclude that hypertension increases pro-oxidant generation and decreases antioxidant defense, and thereby induces renal oxidative stress in early diabetes. Internal Medicine, Faculty of Medical Sciences, State University of Campinas, Campinas, Sao Paulo, Brazil. |
NOTE: Color highlight is limited to the abstract and SciMiner text-mining mode. If you see much more identified targets below from "Targets by SciMiner Summary" and "Targets by SciMiner Full list", they may have been identified from the full text.
Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | 2 | nadph oxidase | |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | 1 | gp91phox | |
| 11181 | SOD3 | superoxide dismutase 3, extracellular | 1 | extracellular superoxide dismutase | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | The expression of the gp91phox subunit of NADPH oxidase was significantly elevated in the SHR-D |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | results: nadph oxidase dependent superoxide generation in the renal cortex was significantly elevated in wky and shr diabetic d groups compared to the respective control c groups p _lt_ 0.005 n = 5 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the expression of the gp91phox subunit of nadph oxidase was significantly elevated in the shr d p _lt_ 0.05 n = 5 but not in the wky d group compared to the respective control groups. |
| 11181 | SOD3 | superoxide dismutase 3, extracellular | extracellular superoxide dismutase | 1.0 | the renal cortical extracellular superoxide dismutase level was found to be markedly decreased in the shr groups compared to the wky groups p _lt_ 0.05 n = 5 . |