)| PMID |
14514646 ( ) |
|---|---|
| Title | Translocation of glomerular p47phox and p67phox by protein kinase C-beta activation is required for oxidative stress in diabetic nephropathy. |
| Abstract | Oxidative stress is implicated to play an important role in the development of diabetic vascular complications, including diabetic nephropathy. It is unclear whether oxidative stress is primarily enhanced in the diabetic glomeruli or whether it is merely a consequence of diabetes-induced glomerular injury. To address this issue, we examined diabetic glomeruli to determine whether oxidative stress is enhanced, as well as examined the role of protein kinase C (PKC)-beta activation in modulating NADPH oxidase activity. Urinary 8-hydroxydeoxyguanosine excretion and its intense immune-reactive staining in the glomeruli were markedly higher in diabetic than in control rats, and these alterations were ameliorated by a treatment with a selective PKC-beta inhibitor, ruboxistaurin (RBX; LY333531) mesylate, without affecting glycemia. NADPH oxidase activity, which was significantly enhanced in diabetic glomeruli and the source of reactive oxygen species (ROS) generation, was also improved by RBX treatment by preventing the membranous translocation of p47phox and p67phox from cytoplasmic fraction without affecting their protein levels. Adenoviral-mediated PKC-beta(2) overexpression enhanced ROS generation by modulating the membranous translocation of p47phox and p67phox in cultured mesangial cells. We now demonstrate that oxidative stress is primarily enhanced in the diabetic glomeruli due to a PKC-beta-dependent activation of NADPH oxidase resulting in ROS generation. Japan. |
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Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | 47 | p47phox | |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | 46 | p67phox | |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | 39 | nadph oxidase | |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | 15 | gp91phox | |
| 6081 | INS | insulin | 12 | insulin | |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | 11 | p22phox | |
| 9393 | PRKCA | protein kinase C, alpha | 5 | protein kinase c | PKC-alpha | pkc alpha | |
| 12805 | XDH | xanthine dehydrogenase | 3 | xanthine oxidase | |
| 5013 | HMOX1 | heme oxygenase (decycling) 1 | 1 | heme oxygenase 1 | |
| 8975 | PIK3CA | phosphoinositide-3-kinase, catalytic, alpha polypeptide | 1 | phosphatidylinositol 3 kinase | |
| 2707 | ACE | angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 | 1 | ACE | |
| 9391 | PRKAR2A | protein kinase, cAMP-dependent, regulatory, type II, alpha | 1 | protein kinase a | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | improved by RBX treatment by preventing the membranous translocation of p47phox and p67phox from cytoplasmic fraction without affecting their protein levels |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | RBX treatment by preventing the membranous translocation of p47phox and p67phox from cytoplasmic fraction without affecting their protein levels |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | overexpression enhanced ROS generation by modulating the membranous translocation of p47phox and p67phox in cultured mesangial cells |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | ROS generation by modulating the membranous translocation of p47phox and p67phox in cultured mesangial cells |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | NADPH oxidase consists of several membrane-bound subunits (gp91phox, gp91phox nox and p22phox and cytosolic subunits (p47phox p47phox and p67phox |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | subunits (gp91phox, gp91phox nox and p22phox and cytosolic subunits (p47phox p47phox and p67phox |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | gp91phox nox and p22phox and cytosolic subunits (p47phox p47phox and p67phox |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.0 | oxidase consists of several membrane-bound subunits (gp91phox, gp91phox nox and p22phox and cytosolic subunits (p47phox p47phox and p67phox |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | It has become evident that there are other gp91phox homologues gp91phox is expressed in endothelial cells and adventitial cells |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | It has become evident that there are other gp91phox homologues gp91phox is expressed in endothelial cells and adventitial cells nox-1 is |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | and/or and or protein expression of NADPH oxidase subunits (p47phox, p47phox p67phox p22phox gp91phox nox-1 and nox-4 measurement of NADPH oxidase |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | and or protein expression of NADPH oxidase subunits (p47phox, p47phox p67phox p22phox gp91phox nox-1 and nox-4 measurement of NADPH oxidase activity |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | protein expression of NADPH oxidase subunits (p47phox, p47phox p67phox p22phox gp91phox nox-1 and nox-4 measurement of NADPH oxidase activity |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.5 | or protein expression of NADPH oxidase subunits (p47phox, p47phox p67phox p22phox gp91phox nox-1 and nox-4 measurement of NADPH oxidase activity |
| 2707 | ACE | angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 | ACE | 0.0 | The sections were stained with ACE solution for 20 min at room temperature and washed with |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | For Northern blot analysis of NADPH oxidase subunit consisting of p47phox p67phox p22phox gp91phox nox-1 and nox-4 total RNA was extracted |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Northern blot analysis of NADPH oxidase subunit consisting of p47phox p67phox p22phox gp91phox nox-1 and nox-4 total RNA was extracted from |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | analysis of NADPH oxidase subunit consisting of p47phox p67phox p22phox gp91phox nox-1 and nox-4 total RNA was extracted from isolated glomeruli |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.5 | blot analysis of NADPH oxidase subunit consisting of p47phox p67phox p22phox gp91phox nox-1 and nox-4 total RNA was extracted from isolated |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | alpha -P CTP (New New England Nuclear Boston MA -labeled p47phox p67phox p22phox gp91phox nox-1 and nox-4 cDNA in a buffer |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | -P CTP (New New England Nuclear Boston MA -labeled p47phox p67phox p22phox gp91phox nox-1 and nox-4 cDNA in a buffer (Perfecthybri; |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | (New New England Nuclear Boston MA -labeled p47phox p67phox p22phox gp91phox nox-1 and nox-4 cDNA in a buffer (Perfecthybri; Perfecthybri Toyobo |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.5 | CTP (New New England Nuclear Boston MA -labeled p47phox p67phox p22phox gp91phox nox-1 and nox-4 cDNA in a buffer (Perfecthybri; Perfecthybri |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The cDNAs for p47phox p67phox p22phox gp91phox nox-1 and nox-4 were cloned by RT-PCR |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The cDNAs for p47phox p67phox p22phox gp91phox nox-1 and nox-4 were cloned by RT-PCR of |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | The cDNAs for p47phox p67phox p22phox gp91phox nox-1 and nox-4 were cloned by RT-PCR of total RNA |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.5 | The cDNAs for p47phox p67phox p22phox gp91phox nox-1 and nox-4 were cloned by RT-PCR of total |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The protein expression of p47phox and p67phox in glomeruli |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The protein expression of p47phox and p67phox in glomeruli |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The protein expression of p47phox and p67phox was further examined by Western blot analysis as |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The protein expression of p47phox and p67phox was further examined by Western blot analysis as previously described |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The filter was incubated with anti-mouse p47phox antibody (1:500; 1 500 Transduction Laboratories Lexington KY anti-mouse p67phox |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | p47phox antibody (1:500; 1 500 Transduction Laboratories Lexington KY anti-mouse p67phox antibody (1:500; 1 500 Transduction Laboratories in the buffer (TBS |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The protein expression of p47phox and p67phox in mesangial cells was estimated by Western blot |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The protein expression of p47phox and p67phox in mesangial cells was estimated by Western blot analysis |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | We obtained the samples for p47phox and p67phox expression with the same methods described in the |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | We obtained the samples for p47phox and p67phox expression with the same methods described in the protein expression |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | with the same methods described in the protein expression of p47phox and p67phox in glomeruli |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | same methods described in the protein expression of p47phox and p67phox in glomeruli |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Our data show that not only overexpression of p47phox and p67phox but also their translocation to the membrane are |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Our data show that not only overexpression of p47phox and p67phox but also their translocation to the membrane are correlated with |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Furthermore we demonstrated that membranous translocation of p47phox and p67phox was dependent on the PKC-_amp_#223 activation |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Furthermore we demonstrated that membranous translocation of p47phox and p67phox was dependent on the PKC-_amp_#223 activation |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | One is overexpression of NADPH oxidase subunits including gp91phox gp91phox homologues (nox-1 nox-1 and nox-4 gp22phox gp47phox and gp67phox |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | One is overexpression of NADPH oxidase subunits including gp91phox gp91phox homologues (nox-1 nox-1 and nox-4 gp22phox gp47phox and gp67phox |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Recently the expression of NADPH oxidase subunits (p22phox, p22phox p67phox gp91phox etc. has been shown to be upregulated in several |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | Recently the expression of NADPH oxidase subunits (p22phox, p22phox p67phox gp91phox etc. has been shown to be upregulated in several tissues |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.3 | Recently the expression of NADPH oxidase subunits (p22phox, p22phox p67phox gp91phox etc. has been shown to be upregulated in |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | In diabetic rats renal expression of p47phox when evaluated by Western blot analysis and immunohistochemistry has been |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | by using isolated glomeruli and demonstrating that the expression of p47phox and p67phox was enhanced in the glomeruli of diabetic rats |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | isolated glomeruli and demonstrating that the expression of p47phox and p67phox was enhanced in the glomeruli of diabetic rats |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | Other subunits such as gp91phox nox-1 and p22phox did not differ between control and diabetic |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.0 | Other subunits such as gp91phox nox-1 and p22phox did not differ between control and diabetic rats and nox-4 |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The physiological relevance of increased p47phox and p67phox expression in the induction of oxidative stress is |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The physiological relevance of increased p47phox and p67phox expression in the induction of oxidative stress is supported by |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | We also demonstrated that the enhanced expression of p47phox and p67phox is normalized by insulin but not by RBX |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | We also demonstrated that the enhanced expression of p47phox and p67phox is normalized by insulin but not by RBX |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | These results indicate that the increased p47phox and p67phox expression was mediated by hyperglycemia per se rather |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | These results indicate that the increased p47phox and p67phox expression was mediated by hyperglycemia per se rather than by |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Our present data clearly suggest that the membranous translocation of p47phox and p67phox via PKC-_amp_#223 activation in diabetic glomeruli has a |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | data clearly suggest that the membranous translocation of p47phox and p67phox via PKC-_amp_#223 activation in diabetic glomeruli has a crucial role |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | adenovirus in glomerular mesangial cells caused the membranous translocation of p47phox and p67phox without affecting their protein contents and resulted in |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | glomerular mesangial cells caused the membranous translocation of p47phox and p67phox without affecting their protein contents and resulted in increased O |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Similarly the membranous translocation of p47phox and p67phox was only partially although statistically significantly inhibited |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Similarly the membranous translocation of p47phox and p67phox was only partially although statistically significantly inhibited |
| 9393 | PRKCA | protein kinase C, alpha | PKC-alpha | 1.2 | ( 53 have also shown that PKC-alpha -delta and -zeta can also phosphorylate p47phox and induce its |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | also shown that PKC-alpha -delta and -zeta can also phosphorylate p47phox and induce its membranous translocation |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | and kinases might be associated with the membranous translocation of p47phox and p67phox resulting in oxidative stress |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | might be associated with the membranous translocation of p47phox and p67phox resulting in oxidative stress |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | NADPH oxidase activation at least in part through PKC-_amp_#223 -dependent p47phox and p67phox membranous translocation |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | activation at least in part through PKC-_amp_#223 -dependent p47phox and p67phox membranous translocation |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The mRNA expression of NADPH oxidase subunits consisting of p47phox ( A p67phox ( B nox-4( nox-4 C gp91phox ( |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | expression of NADPH oxidase subunits consisting of p47phox ( A p67phox ( B nox-4( nox-4 C gp91phox ( D nox-1 ( |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | of p47phox ( A p67phox ( B nox-4( nox-4 C gp91phox ( D nox-1 ( E and p22phox ( F and |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | E and p22phox ( F and the protein expression of p47phox ( G and p67phox ( H in glomeruli |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | F and the protein expression of p47phox ( G and p67phox ( H in glomeruli |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.0 | nox-4( nox-4 C gp91phox ( D nox-1 ( E and p22phox ( F and the protein expression of p47phox ( G |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | results of Western blot analysis for total glomerular lysates of p47phox and p67phox obtained from two independent samples are shown in |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Western blot analysis for total glomerular lysates of p47phox and p67phox obtained from two independent samples are shown in the upper |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Effect of PKC-_amp_#223 inhibition on p47phox and p67phox expression and their membranous translocation in diabetic glomeruli |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Effect of PKC-_amp_#223 inhibition on p47phox and p67phox expression and their membranous translocation in diabetic glomeruli |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | results of Western blot analysis for total glomerular lysates of p47phox and p67phox obtained from two independent samples are shown in |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Western blot analysis for total glomerular lysates of p47phox and p67phox obtained from two independent samples are shown in the upper |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Western blot analysis for the membranous and cytosolic fraction of p47phox and p67phox are shown in the upper panel |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | analysis for the membranous and cytosolic fraction of p47phox and p67phox are shown in the upper panel |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Adenovirus-induced overexpression of PKC-_amp_#223 2 causes the membranous translocation of p47phox and p67phox |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | of PKC-_amp_#223 2 causes the membranous translocation of p47phox and p67phox |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | for the membranous cytosolic fraction and total cell lysates of p47phox and p67phox are shown in the upper panel |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | membranous cytosolic fraction and total cell lysates of p47phox and p67phox are shown in the upper panel |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | The mRNA expression of NADPH oxidase subunits consisting of gp91phox nox-1 nox-4 p22phox p47phox and p67phox in glomeruli was evaluated |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | of NADPH oxidase subunits consisting of gp91phox nox-1 nox-4 p22phox p47phox and p67phox in glomeruli was evaluated by Northern blot analysis |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | oxidase subunits consisting of gp91phox nox-1 nox-4 p22phox p47phox and p67phox in glomeruli was evaluated by Northern blot analysis |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.2 | expression of NADPH oxidase subunits consisting of gp91phox nox-1 nox-4 p22phox p47phox and p67phox in glomeruli was evaluated by Northern blot |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Among these subunits the mRNA expression of p47phox and p67phox was significantly upregulated in diabetic glomeruli ( Fig |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | Among these subunits the mRNA expression of p47phox and p67phox was significantly upregulated in diabetic glomeruli ( Fig 4 A |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | in diabetic glomeruli ( Fig 4 A and B whereas gp91phox p22phox and nox-1 expression was unchanged ( Fig 4 D |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.2 | diabetic glomeruli ( Fig 4 A and B whereas gp91phox p22phox and nox-1 expression was unchanged ( Fig 4 D -F |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | The protein expression of p47phox and p67phox estimated by Western blot analysis was also upregulated |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | The protein expression of p47phox and p67phox estimated by Western blot analysis was also upregulated in diabetic |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | These alterations in p47phox p67phox and nox-4 expression were normalized by insulin ( Fig |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | These alterations in p47phox p67phox and nox-4 expression were normalized by insulin ( Fig 4 |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 1.0 | A -C G and H and the mRNA expression of gp91phox p22phox and nox-1 was unaltered by insulin (data data not |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.2 | -C G and H and the mRNA expression of gp91phox p22phox and nox-1 was unaltered by insulin (data data not shown |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | Effect of PKC-_amp_#223 inhibition on mRNA and protein expression of p47phox and p67phox components and on membranous translocation of p47phox and |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | PKC-_amp_#223 inhibition on mRNA and protein expression of p47phox and p67phox components and on membranous translocation of p47phox and p67phox in |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | of p47phox and p67phox components and on membranous translocation of p47phox and p67phox in vivo |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | and p67phox components and on membranous translocation of p47phox and p67phox in vivo |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | we examined the effect of PKC-_amp_#223 inhibition with RBX on p47phox and p67phox overexpression in diabetic glomeruli |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | the effect of PKC-_amp_#223 inhibition with RBX on p47phox and p67phox overexpression in diabetic glomeruli |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | RBX failed to normalize the mRNA and protein expression of p47phox and p67phox protein using total cell lysates ( Fig 4 |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | to normalize the mRNA and protein expression of p47phox and p67phox protein using total cell lysates ( Fig 4 A and |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | However the increased membranous translocation of p47phox and p67phox in diabetic glomeruli was significantly suppressed by PKC-_amp_#223 |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | However the increased membranous translocation of p47phox and p67phox in diabetic glomeruli was significantly suppressed by PKC-_amp_#223 inhibition with |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | on O 2 production is dependent on membranous translocation of p47phox and p67phox |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | 2 production is dependent on membranous translocation of p47phox and p67phox |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | used an in vitro system to evaluate membranous translocation of p47phox and p67phox in rat mesangial cells |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | in vitro system to evaluate membranous translocation of p47phox and p67phox in rat mesangial cells |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | In a similar system the membranous translocation of p47phox and p67phox was significantly enhanced by PKC-_amp_#223 2 overexpression compared |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | In a similar system the membranous translocation of p47phox and p67phox was significantly enhanced by PKC-_amp_#223 2 overexpression compared with GFP |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 4.9 | 2 overexpression compared with GFP overexpression whereas the expression of p47phox and p67phox in the cytoplasmic fraction and total cell lysates |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 2.7 | compared with GFP overexpression whereas the expression of p47phox and p67phox in the cytoplasmic fraction and total cell lysates did not |
| 9393 | PRKCA | protein kinase C, alpha | protein kinase c | 1.0 | to address this issue we examined diabetic glomeruli to determine whether oxidative stress is enhanced as well as examined the role of protein kinase c pkc _amp_#223; activation in modulating nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | to address this issue we examined diabetic glomeruli to determine whether oxidative stress is enhanced as well as examined the role of protein kinase c pkc _amp_#223; activation in modulating nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | nadph oxidase activity which was significantly enhanced in diabetic glomeruli and the source of reactive oxygen species ros generation was also improved by rbx treatment by preventing the membranous translocation |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | we now demonstrate that oxidative stress is primarily enhanced in the diabetic glomeruli due to a pkc _amp_#223; dependent activation of nadph oxidase resulting in ros generation. |
| 5013 | HMOX1 | heme oxygenase (decycling) 1 | heme oxygenase 1 | 1.0 | we have also reported that the expression of hemox1 heme oxygenase 1 a redox sensitive and inducible gene 9 is enhanced in the glomeruli of rats with streptozotocin induced diabetes 10 . |
| 9393 | PRKCA | protein kinase C, alpha | protein kinase c | 1.0 | in diabetes reactive oxygen species ros is thought to be generated through the increased formation of advanced glycation end products 3 increased polyol pathway 11 mitochondrial dysfunction 12 and protein kinase c pkc activation 13 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | numerous reports have recently shown that nadph oxidase which is primarily found in phagocytic cells is the main source of ros in nonphagocytic cells such as endothelial cells 14 adventitial cells 15 smooth muscle cells 16 17 mesangial cells 18 podocytes |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | nadph oxidase consists of several membrane bound subunits gp91phox nox and p22phox and cytosolic subunits p47phox and p67phox . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | pkc is one of the regulators of nadph oxidase activation in neutrophils 22 ; however its molecular mechanism in enhancing nadph oxidase activity in nonphagocytic cells remains unknown. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the aims of the present study were to determine whether oxidative stress is primarily enhanced in the diabetic glomeruli and to determine the role of nadph oxidase in the generation of ros. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | we also examined the underlying molecular mechanism by which pkc _amp_#223; activation might modulate nadph oxidase in diabetic rats and in glomerular mesangial cells. |
| 6081 | INS | insulin | insulin | 1.0 | rats were divided into the following groups: control rats diabetic rats diabetic rats treated with implantation of insulin pellets linshin scarborough on canada subcutaneously and diabetic rats treated with a selective pkc _amp_#223; inhibitor ruboxistaurin rbx; ly333531 mesylate 10 mg _amp_#183; kg body wt _amp_#183; da |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | renal glomeruli were isolated by sieving with stainless steel and nylon meshes as previously described 26 for mrna and/or protein expression of nadph oxidase subunits p47phox p67phox p22phox gp91phox nox 1 and nox 4 measurement of nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | subunits p47phox p67phox p22phox gp91phox nox 1 and nox 4 measurement of nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | estimation of nadph oxidase activity. |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | l/l dpi 10 micromol/l indomethacin an inhibitor of cyclooxygenase 10 micromol/l n g monomethyl l arginine l nmma; an inhibitor of nitric oxide [no] synthase 100 micromol/l allopurinol an inhibitor of xanthine oxidase and 10 mmol/l tiron. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the mrna expression of nadph oxidase subunit. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | for northern blot analysis of nadph oxidase subunit consisting of p47phox p67phox p22phox gp91phox nox 1 and nox 4 total rna was extracted from isolated glomeruli using a commercial preparation based on guanidinium and phenol extraction trizol |
| 6081 | INS | insulin | insulin | 1.0 | in rats treated with insulin blood glucose was normalized. |
| 6081 | INS | insulin | insulin | 1.0 | body weight was significantly lower and kidney weight greater in diabetic compared with control rats and insulin treatment again normalized these alterations. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | our data show that not only overexpression of p47phox and p67phox but also their translocation to the membrane are correlated with the overall increased nadph oxidase activity resulting in oxidative stress in diabetic glomeruli. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | production of o 2 was significantly increased in the diabetic glomeruli and this enhancement was inhibited by dpi a nadph oxidase inhibitor. |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | however dpi can inhibit other flavoprotein containing enzymes such as xanthine oxidase no synthase cyclooxygenase and mitochondrial electron transport chain. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the present study we used the non redox cycling dose of lucigenin at the concentration of 5 micromol/l suggesting that the observed o 2 production in glomerular homogenates correlated specifically to nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the nonphagocytic nadph oxidase which is constitutively active normally produces relatively low levels of ros under basal conditions. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | one is overexpression of nadph oxidase subunits including gp91phox gp91phox homologues nox 1 and nox 4 gp22phox gp47phox and gp67phox. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | recently the expression of nadph oxidase subunits p22phox p67phox gp91phox etc. has been shown to be upregulated in several tissues in cardiovascular diseases 32 47 49 . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | to understand the ability of diabetes and/or hyperglycemia to regulate the gene expression of nadph oxidase subunits and their action requires further studies. |
| 6081 | INS | insulin | insulin | 1.0 | we also demonstrated that the enhanced expression of p47phox and p67phox is normalized by insulin but not by rbx. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | although pkc has been shown to be one of the regulators of nadph oxidase activation in phagocytic cells there are few reports in nonphagocytic cells. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | however they failed to measure oxidase activity or to investigate the expression of nadph oxidase subunits. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | it should be noted that the link between diabetes pkc _amp_#223; activation nadph oxidase activity and oxidative stress to the best of our knowledge has not been previously reported in vascular tissues and cells. |
| 8975 | PIK3CA | phosphoinositide-3-kinase, catalytic, alpha polypeptide | phosphatidylinositol 3 kinase | 1.0 | the membranous translocation can be regulated by not only pkc_amp_#223; but also by other pkc isoforms: protein kinase a mitogen activated kinases and phosphatidylinositol 3 kinase 22 51 52 . |
| 9391 | PRKAR2A | protein kinase, cAMP-dependent, regulatory, type II, alpha | protein kinase a | 1.0 | the membranous translocation can be regulated by not only pkc_amp_#223; but also by other pkc isoforms: protein kinase a mitogen activated kinases and phosphatidylinositol 3 kinase 22 51 52 . |
| 9393 | PRKCA | protein kinase C, alpha | pkc alpha | 1.0 | 53 have also shown that pkc alpha delta and zeta can also phosphorylate p47phox and induce its membranous translocation. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | in conclusion the present study provides evidence that oxidative stress in the diabetic glomeruli in the early course of diabetes is mediated by nadph oxidase activation at least in part through pkc _amp_#223; dependent p47phox and p67phox membranous translocation. |
| 6081 | INS | insulin | insulin | 1.0 | representative results of immunoreactive expression of renal 8 ohdg from control a diabetic b diabetic plus insulin c and diabetic plus rbx rbx mesylate d are shown. |
| 6081 | INS | insulin | insulin | 1.0 | nadph 100 micromol/l nadh 100 micromol/l arachidonic acid aa; 100 micromol/l succinate sa; 5 mmol/l or xanthine xa; 100 nmol/l is added to glomerular homogenates from control diabetic diabetic plus insulin or diabetic plus rbx rbx mesylate . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the effect of diabetes on nadph oxidase subunits in vivo. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the mrna expression of nadph oxidase subunits consisting of p47phox a p67phox b nox 4 c gp91phox d nox 1 e and p22phox f and the protein expression of p47phox g and p67phox h in glomeruli. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | a f : representative results of northern blot analysis of nadph oxidase subunits and 36b4 are shown in the upper panel. |
| 6081 | INS | insulin | insulin | 1.0 | data are expressed as means _amp_#177; se n = 4 * p _lt_ 0.05 vs control and diabetic plus insulin groups . |
| 6081 | INS | insulin | insulin | 1.0 | the increased urinary 8 ohdg excretion in diabetic rats was completely normalized by treatment with insulin and significantly ameliorated by rbx table 2 . |
| 6081 | INS | insulin | insulin | 1.0 | aining of 8 ohdg was markedly increased in the nuclei of diabetic glomerular cells such as mesangial cell endothelial cell and podocyte compared with those from control and diabetic rats treated with insulin fig 1 a c . |
| 6081 | INS | insulin | insulin | 1.0 | normalization of glycemia by insulin completely normalized increased o 2 production 630.9 _amp_#177; 28.3 cpm _amp_#183; min _amp_#183; mg protein n = 4 ns vs control . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | in addition the enhanced lucigenin signal in diabetic glomeruli was significantly inhibited by treating with 10 micromol/l dpi a specific inhibitor of nadph oxidase and 10 mmol/l tiron an o 2 scavenger. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | pkc _amp_#223; inhibition suppresses diabetes induced glomerular nadph oxidase activation. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | as shown in fig. 3 a o 2 production derived from nadph oxidase was greater than that from other potential substrates including nadh xanthine succinate or arachidonic acid. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | nadph oxidase activity was significantly enhanced in the diabetic glomerular homogenates compared with those from controls 4 296.0 _amp_#177; 75.8 vs 2 399.4 _amp_#177; 79.8 cpm _amp_#183; min _amp_#183; mg protei |
| 6081 | INS | insulin | insulin | 1.0 | this enhanced activation of nadph oxidase was normalized by insulin 2 574.6 _amp_#177; 54.7 cpm _amp_#183; min _amp_#183; mg protein n = 5 p _lt_ 0.05 vs diabetic . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | this enhanced activation of nadph oxidase was normalized by insulin 2 574.6 _amp_#177; 54.7 cpm _amp_#183; min _amp_#183; mg protein n = 5 p _lt_ 0.05 vs diabetic . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | pkc _amp_#223; inhibition with rbx significantly suppressed nadph oxidase activation 3 432.6 _amp_#177; 152.2 cpm _amp_#183; min _amp_#183; mg protein n = 5 p _lt_ 0.05 vs diabetic fig 3 a . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | as shown in fig. 3 b nadph oxidase activity was completely blocked by dpi and tiron whereas rotenone an inhibitor of the electron transport chain allopurinol an inhibitor of xanthine oxidase indomethacin a cyclooxygenase inhibitor and |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | as shown in fig. 3 b nadph oxidase activity was completely blocked by dpi and tiron whereas rotenone an inhibitor of the electron transport chain allopurinol an inhibitor of xanthine oxidase indomethacin a cyclooxygenase inhibitor and l nmma a no synthase inhibitor had no effect. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | this appears to indicate that the main enzyme source for production of o 2 in all the glomeruli from control and diabetic rats is derived from nadph oxidase activity. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the effect of diabetes on nadph oxidase subunits in vivo. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | the mrna expression of nadph oxidase subunits consisting of gp91phox nox 1 nox 4 p22phox p47phox and p67phox in glomeruli was evaluated by northern blot analysis. |
| 6081 | INS | insulin | insulin | 1.0 | these alterations in p47phox p67phox and nox 4 expression were normalized by insulin fig 4 a c g and h and the mrna expression of gp91phox p22phox and nox 1 was unaltered by insulin data not shown . |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | to determine whether the pkc _amp_#223; which has been shown to be activated in diabetic glomeruli 23 24 contributes to the nadph oxidase activation we examined the effect of pkc _amp_#223; inhibition with rbx on p47phox and p67phox overexpression in diabetic glomeruli. |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | to delineate the underlying mechanism by which pkc _amp_#223; could modulate nadph oxidase we used an in vitro system to evaluate membranous translocation of p47phox and p67phox in rat mesangial cells. |
| 9393 | PRKCA | protein kinase C, alpha | protein kinase c | 1.0 | abbreviations: 8 ohdg 8 hydroxydeoxyguanosine; dpi diphenylene iodonium; gfp green fluorescent protein; l nmma n g monomethyl l arginine; nih national institutes of health; pkc protein kinase c; rbx ruboxistaurin; ros reactive oxygen species; tbs tris buffered saline |