)| PMID |
12639760 ( ) |
|---|---|
| Title | Enhanced vascular production of superoxide in OLETF rat after the onset of hyperglycemia. |
| Abstract | This study was aimed to characterize the vascular production of superoxide in Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model of type 2 diabetes. The nitroblue tetrazolium staining in the aorta from old (30 weeks) OLETF rat was more prominent than that of age-matched control (LETO) rat, which was significantly inhibited by diphenyleneiodonium (10 micromol/l), but not by inhibitors for other oxidases such as xanthine oxidase, mitochondrial oxidase, nitric oxide synthase, and cyclooxygenase. In the aorta from old OLETF rat with hyperglycemia, the enhanced NADH oxidase activity in association with upregulated expression of p22phox and gp91phox was observed, but not in both LETO and young (10 weeks) OLETF rats without hyperglycemia. Furthermore, there was a positive correlation (P<0.01) between elevation of blood glucose level and increase in vascular NADH oxidase activity. Based on these results, it was suggested that the enhanced NADH oxidase activity in the aorta from OLETF rat occurred after the onset of hyperglycemia, thereby resulting in the increased vascular production of superoxide. Pusan National University, Busan 602-739, South Korea. |
NOTE: Color highlight is limited to the abstract and SciMiner text-mining mode. If you see much more identified targets below from "Targets by SciMiner Summary" and "Targets by SciMiner Full list", they may have been identified from the full text.
Targets by SciMiner Summary
| HUGO ID | Symbol | Target Name | #Occur | ActualStr |
|---|---|---|---|---|
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | 13 | gp91phox | |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | 13 | p22phox | |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | 7 | SOD | |
| 7873 | NOS2A | nitric oxide synthase 2A (inducible, hepatocytes) | 3 | nitric oxide synthase | |
| 12805 | XDH | xanthine dehydrogenase | 3 | xanthine oxidase | |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | 2 | nadph oxidase | |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | 1 | p67phox | |
| 391 | AKT1 | v-akt murine thymoma viral oncogene homolog 1 | 1 | rac | |
| 9393 | PRKCA | protein kinase C, alpha | 1 | protein kinase c | |
| 333 | AGT | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) | 1 | angiotensin ii | |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | 1 | p47phox | |
Targets by SciMiner Full list
| HUGO ID | Symbol | Name | ActualStr | Score | FlankingText |
|---|---|---|---|---|---|
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | enhanced NADH oxidase activity in association with upregulated expression of p22phox and gp91phox was observed but not in both LETO and |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | oxidase activity in association with upregulated expression of p22phox and gp91phox was observed but not in both LETO and young (10 |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | incubated for 30 min in the presence or absence of SOD (200 200 U/ml), U ml tiron (10 10 mM diphenyleneiodonium |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | Western blot for p22phox and gp91phox |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | Western blot for p22phox and gp91phox |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | milk the transferred membrane was then incubated with antibodies against p22phox and gp91phox (goat goat polyclonal antibody Santa Cruz Biotech |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | transferred membrane was then incubated with antibodies against p22phox and gp91phox (goat goat polyclonal antibody Santa Cruz Biotech |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | The superoxide scavengers SOD (200 200 U/ml) U ml and tiron (10 10 mmol/l), |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | The effect of tiron was greater than that of SOD 3.3 |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | Expression of p22phox and gp91phox |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | Expression of p22phox and gp91phox |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | We examined protein expression for p22phox and gp91phox in the aorta from old OLETF rat by |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | We examined protein expression for p22phox and gp91phox in the aorta from old OLETF rat by Western blot |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | As shown in Fig 5 p22phox and gp91phox protein expression in the vasculature from OLETF rat |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | As shown in Fig 5 p22phox and gp91phox protein expression in the vasculature from OLETF rat was significantly |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | enhanced activity of vascular NADH oxidase with upregulated expression of p22phox and gp91phox was evident in the aorta from old OLETF |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | of vascular NADH oxidase with upregulated expression of p22phox and gp91phox was evident in the aorta from old OLETF rat (4) |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | assay was further indicated by showing that the superoxide scavengers SOD and tiron decreased the reduction of NBT |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | In our experiment SOD decreased NBT reduction to a lesser extent than did tiron |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | In contrast at least in the short term exogenous SOD only has access to superoxide generated extracellularly since it is |
| 7660 | NCF1 | neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) | p47phox | 1.0 | H oxidase complex is composed of several cytoplasmic factors (p47phox, p47phox p67phox and rac and a membrane-bound flavocytochrome b558 consisting of |
| 7661 | NCF2 | neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2) | p67phox | 1.0 | oxidase complex is composed of several cytoplasmic factors (p47phox, p47phox p67phox and rac and a membrane-bound flavocytochrome b558 consisting of a |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | membrane-bound flavocytochrome b558 consisting of a small _amp_#x3b1 -subunit (p22phox) p22phox and a larger glycosylated _amp_#x3b2 -subunit gp91phox 19 20 21 |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | _amp_#x3b1 -subunit (p22phox) p22phox and a larger glycosylated _amp_#x3b2 -subunit gp91phox 19 20 21 22 and 23 |
| 391 | AKT1 | v-akt murine thymoma viral oncogene homolog 1 | rac | 0.1 | is composed of several cytoplasmic factors (p47phox, p47phox p67phox and rac and a membrane-bound flavocytochrome b558 consisting of a small _amp_#x3b1 |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | membrane-bound subunits of NAD(P)H NAD P H oxidase such as gp91phox and p22phox are essential for the stability of oxidase and |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | of NAD(P)H NAD P H oxidase such as gp91phox and p22phox are essential for the stability of oxidase and its catalytic |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | activation in OLETF rat we determined the protein expression of gp91phox and p22phox by Western blot analysis |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | OLETF rat we determined the protein expression of gp91phox and p22phox by Western blot analysis |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | Our results clearly showed that the expression of both p22phox and gp91phox in the aorta from OLETF rat was significantly |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | results clearly showed that the expression of both p22phox and gp91phox in the aorta from OLETF rat was significantly higher than |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | In summary upregulated expression of p22phox and gp91phox enhanced NADH oxidase activity subsequently causing the accelerated |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | In summary upregulated expression of p22phox and gp91phox enhanced NADH oxidase activity subsequently causing the accelerated adventitial production |
| 11179 | SOD1 | superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | SOD | 0.3 | several inhibitors such as tiron (10 10 mmol/l), mmol l SOD (200 200 U/ml), U ml DPI (10 10 _amp_#x3bc;mol/l), _amp_#x3bc |
| 2577 | CYBA | cytochrome b-245, alpha polypeptide | p22phox | 0.8 | Representative Western blots analysis for p22phox gp91phox and _amp_#x3b2 -actin in the aortas from LETO and |
| 2578 | CYBB | cytochrome b-245, beta polypeptide (chronic granulomatous disease) | gp91phox | 2.3 | Representative Western blots analysis for p22phox gp91phox and _amp_#x3b2 -actin in the aortas from LETO and OLETF |
| 7873 | NOS2A | nitric oxide synthase 2A (inducible, hepatocytes) | nitric oxide synthase | 1.0 | at of age matched control leto rat which was significantly inhibited by diphenyleneiodonium 10 _amp_#x3bc;mol/l but not by inhibitors for other oxidases such as xanthine oxidase mitochondrial oxidase nitric oxide synthase and cyclooxygenase. |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | ks oletf rat was more prominent than that of age matched control leto rat which was significantly inhibited by diphenyleneiodonium 10 _amp_#x3bc;mol/l but not by inhibitors for other oxidases such as xanthine oxidase mitochondrial oxidase nitric oxide synthase and cyclooxygenase. |
| 7873 | NOS2A | nitric oxide synthase 2A (inducible, hepatocytes) | nitric oxide synthase | 1.0 | dpi 10 _amp_#x3bc;mol/l but not by other inhibitors such as allopurinol xanthine oxidase inhibitor 300 _amp_#x3bc;mol/l rotenone inhibitor of mitochondrial electron transport 50 _amp_#x3bc;mol/l nmma nitric oxide synthase inhibitor 300 _amp_#x3bc;mol/l and indomethacin cyclooxygenase inhibitor 100 _amp_#x3bc;mol/l . |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | the increased nbt reduction in the aorta from oletf rat was greatly reduced by treatment with an nad p h oxidase inhibitor dpi 10 _amp_#x3bc;mol/l but not by other inhibitors such as allopurinol xanthine oxidase inhibitor 300 _amp_#x3bc;mol/l rotenone inhibitor of mitochondrial electron transport 50 _amp_#x3bc;mol/l nmma nitric oxide synthase inhibitor 300 _amp_#x3bc;mol/l and indomethacin cyclooxygenase inh |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | recently in angiotensin ii induced hypertension wang et al. [ 16 ] demonstrated that the production of superoxide and the expression of nadph oxidase subunits remain primarily localized in the adventitia and adventitial superoxide played a paracrine role in mediating spontaneous tone of the aorta. |
| 333 | AGT | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) | angiotensin ii | 1.0 | recently in angiotensin ii induced hypertension wang et al. [ 16 ] demonstrated that the production of superoxide and the expression of nadph oxidase subunits remain primarily localized in the adventitia and adventitial supero |
| 7873 | NOS2A | nitric oxide synthase 2A (inducible, hepatocytes) | nitric oxide synthase | 1.0 | ction in the aorta from oletf rat was significantly reduced by treatment with dpi an nad p h oxidase inhibitor but not by inhibitors for other oxidases such as xanthine oxidase mitochondrial oxidases nitric oxide synthase and cyclooxygenase suggesting that nad p h oxidase is involved in the vascular production of superoxide in oletf rat. |
| 12805 | XDH | xanthine dehydrogenase | xanthine oxidase | 1.0 | the enhanced nbt reduction in the aorta from oletf rat was significantly reduced by treatment with dpi an nad p h oxidase inhibitor but not by inhibitors for other oxidases such as xanthine oxidase mitochondrial oxidases nitric oxide synthase and cyclooxygenase suggesting that nad p h oxidase is involved in the vascular production of superoxide in oletf rat. |
| 9393 | PRKCA | protein kinase C, alpha | protein kinase c | 1.0 | based on these results with others that high glucose stimulates the production of ros through protein kinase c dependent activation of nad p h oxidase in vascular smooth muscle cells [ 26 ] it is likely that the enhanced activity of vascular nadh oxidase in old oletf rat is closely related with hyperglycemia |
| 14874 | NOX5 | NADPH oxidase, EF-hand calcium binding domain 5 | nadph oxidase | 1.0 | cybb protein human|membrane glycoproteins|membrane transport proteins|multienzyme complexes|phosphoproteins|superoxides|nadh oxidase|nadh nadph oxidoreductases|cyba protein human|nadph oxidase|nadph dehydrogenase| |